Human/Mouse/Rat APBA2 Antibody

Catalog #: AF6327 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6327
AF6327-SP

Detection of Human APBA2 by Western Blot.

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Human/Mouse/Rat APBA2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human APBA2 in direct ELISAs and human, mouse and rat in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) APBA1 and rhAPBA3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human APBA2
Ala2-Leu165
Accession # Q99767

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human APBA2 by Western Blot. Western blot shows lysates of human brain (cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human APBA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6327) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for APBA2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APBA2

APBA2 (Adaptor protein X11 beta, also Mint2 and X11L) is a cytosolic member of the X11 family of adaptor proteins. Although its predicted MW is 83 kDa, it runs anomalously at 130-135 kDa in SDS-PAGE. APBA2 is expressed in neurons, and appears to play a role in A beta formation from APP. Normally, APBA2 associates with the cytoplasmic domain of transmembrane APP. This precludes its entry into lipid rafts, where it begins formation of A beta following cleaved by BACE. Human APBA2 is 749 amino acids (aa ) in length. It contains one phosphotyrosine binding (PTB) domain (aa 366-533) plus two PDZ domains (aa 568-734). Over aa 221-250, there is an APP interaction site that contains a key phosphorylation site at Ser238. There are two potential splice forms, one that shows a deletion of aa 406-417, and a second that contains a 44 aa substitution for aa 170-214. Over aa 1-165, human APBA2 shares 85% aa identity with mouse APBA2.

Long Name

Amyloid beta (A4) Precursor Protein-binding, Family A, Member 2

Entrez Gene IDs

321 (Human); 11784 (Mouse); 83610 (Rat)

Alternate Names

Amyloid-beta A4 precursor protein-binding family A member 2;D15S1518E;HsT16821;LIN-10;MGC:14091;MINT2;X11-BETA;X11L; APBA2; LIN-10; MINT2; X11L