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Human/Mouse/Rat Axin-1 Antibody

Catalog #: AF3287
15 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3287
AF3287-SP
Detection of Human/Mouse/Rat Axin‑1 by Western Blot.
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Product Details
Citations (15)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Axin-1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Axin-1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Axin-1
Tyr210-Glu410
Accession # O15169
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Axin‑1 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Axin‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axin-1

Axin-1 is a negative regulator of the Wnt signaling pathway, which is involved in regulation of embryonic axis formation in vertebrates. Axin-1 binds to beta -catenin and glycogen synthase kinase-3 (GSK-3) to promote beta -catenin phosphorylation and subsequent degradation. Axin-1 also binds to the adenomatous polyposis coli (APC) gene, a known tumor suppressor that also regulates beta -catenin.

Long Name
Axis Inhibitor 1
Entrez Gene IDs
8312 (Human); 12005 (Mouse); 79257 (Rat)
Alternate Names
axin 1; Axin1; Axin-1; AXINaxis inhibitor 1; Axis inhibition protein 1; fused, mouse, homolog of; hAxin; MGC52315

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Citations for Human/Mouse/Rat Axin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. BK Channel Deficiency in Osteoblasts Reduces Bone Formation via the Wnt/ beta -Catenin Pathway
    Authors: Lan Jiang, Qianhong Yang, Jianjun Gao, Jiahong Yang, Jiaqi He, Hong Xin et al.
    Molecules and Cells
  2. Evaluation of AXIN1 and AXIN2 as targets of tankyrase inhibition in hepatocellular carcinoma cell lines
    Authors: W Wang, P Liu, M Lavrijsen, S Li, R Zhang, S Li, WS van de Gee, HJG van de Wer, MP Peppelenbo, R Smits
    Scientific Reports, 2021-04-02;11(1):7470.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  3. APC controls Wnt-induced beta-catenin destruction complex recruitment in human colonocytes
    Authors: TW Parker, KL Neufeld
    Sci Rep, 2020-02-19;10(1):2957.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Treatment with XAV-939 prevents in vitro calcification of human valvular interstitial cells
    Authors: C Dittfeld, G Reimann, A Mieting, P Büttner, A Jannasch, K Plötze, G Steiner, SM Tugtekin, K Matschke
    PLoS ONE, 2018-12-07;13(12):e0208774.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway
    Authors: K Saito-Diaz, H Benchabane, A Tiwari, A Tian, B Li, JJ Thompson, AS Hyde, LM Sawyer, JN Jodoin, E Santos, LA Lee, RJ Coffey, RD Beauchamp, CS Williams, AK Kenworthy, DJ Robbins, Y Ahmed, E Lee
    Dev. Cell, 2018-03-12;44(5):566-581.e8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling.
    Authors: Lebensohn A, Dubey R, Neitzel L, Tacchelly-Benites O, Yang E, Marceau C, Davis E, Patel B, Bahrami-Nejad Z, Travaglini K, Ahmed Y, Lee E, Carette J, Rohatgi R
    Elife, 2016-12-20;5(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Axin cancer mutants form nanoaggregates to rewire the Wnt signaling network
    Authors: Zeinab Anvarian, Hisashi Nojima, Eline C van Kappel, Tobias Madl, Maureen Spit, Martin Viertler et al.
    Nature Structural & Molecular Biology
  8. Tankyrase Inhibitors Stimulate the Ability of Tankyrases to Bind Axin and Drive Assembly of beta -Catenin Degradation-Competent Axin Puncta
    Authors: Estefania Martino-Echarri, Mariana G. Brocardo, Kate M. Mills, Beric R. Henderson
    PLOS ONE
  9. HSP105 recruits protein phosphatase 2A to dephosphorylate beta-catenin.
    Authors: Yu N, Kakunda M, Pham V, Lill J, Du P, Wongchenko M, Yan Y, Firestein R, Huang X
    Mol Cell Biol, 2015-02-02;35(8):1390-400.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  10. Inhibitors of endocytosis prevent Wnt/Wingless signalling by reducing the level of basal beta –Catenin/Armadillo
    Authors: Maria Gagliardi, Ana Hernandez, Ian J. McGough, Jean-Paul Vincent
    Journal of Cell Science
  11. Activation of Wnt/ beta -Catenin Signaling Increases Apoptosis in Melanoma Cells Treated with Trail
    Authors: Zachary F. Zimmerman, Rima M. Kulikauskas, Karol Bomsztyk, Randall T. Moon, Andy J. Chien
    PLoS ONE
  12. Inhibition of tankyrases induces Axin stabilization and blocks Wnt signalling in breast cancer cells.
    Authors: Bao R, Christova T, Song S, Angers S, Yan X, Attisano L
    PLoS ONE, 2012-11-07;7(11):e48670.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Dual functions of DP1 promote biphasic Wnt-on and Wnt-off states during anteroposterior neural patterning.
    EMBO J., 2012-07-06;31(16):3384-97.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Wnt/ beta -Catenin Signaling and AXIN1 Regulate Apoptosis Triggered by Inhibition of the Mutant Kinase BRAF V600E in Human Melanoma
    Authors: Travis L. Biechele, Rima M. Kulikauskas, Rachel A. Toroni, Olivia M. Lucero, Reyna D. Swift, Richard G. James et al.
    Science Signaling
  15. Canonical Wnt signaling negatively regulates platelet function.
    Authors: Steele BM, Harper MT, Macaulay IC, Morrell CN, Perez-Tamayo A, Foy M, Habas R, Poole AW, Fitzgerald DJ, Maguire PB
    Proc. Natl. Acad. Sci. U.S.A., 2009-11-09;106(47):19836-41.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat Axin-1 Antibody

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Human/Mouse/Rat Axin‑1 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: EMT6 cells Species: Mouse

From left to right are lanes: negative control without or with Wnt3a treatment, and Axin1 stabilizer XAV939 without or with Wnt3a treatment.
Specificity: Specific
Sensitivity: Sensitive
Buffer: TBS+Tween 0.1%
Dilution: 1/1000

Western Blot Human/Mouse/Rat Axin‑1 Antibody AF3287