Human/Mouse/Rat B7-H4 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB21542V Datasheet / COA / SDS

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FAB21542V-100UG

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Human/Mouse/Rat B7-H4 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects mouse B7-H4 in direct ELISAs. Detects human, mouse, and rat B7-H4 in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2582H

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse B7-H4
Phe29-Pro258
Accession # Q7TSP5

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution

Reconstitution default not known

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-H4

B7-H4, also known as B7x and B7S1, belongs to the B7 family of costimulatory proteins and contains two extracellular Ig-like domains. B7-H4 is expressed on T cells, B cells, monocytes/macrophages, dendritic cells, and some tumor cells. B7-H4 signaling inhibits T cell activation, proliferation and maturation.

Long Name

B7 Homolog 4

Entrez Gene IDs

79679 (Human); 242122 (Mouse); 295322 (Rat)

Alternate Names

B7h.5; B7H4; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1; B7S1VCTN1; B7x; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; Vtcn1

Product Datasheets

Product Datasheet

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SDS

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.