Human/Mouse/Rat BTF3 Antibody

Catalog #: AF5747
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5747
AF5747-SP

Detection of Human/Mouse/Rat BTF3 by Western Blot.

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All BTF3 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat BTF3 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat BTF3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BTF3
Lys46-Asn206
Accession # P20290

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat BTF3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SK-BR-3 human breast cancer cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat BTF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5747) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BTF3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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BTF3 in Human Pancreas Cancer Tissue. BTF3 was detected in immersion fixed paraffin-embedded sections of human pancreas cancer tissue using Human/Mouse/Rat BTF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5747) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTF3

BTF3 (basic transcription factor 3; also nascent polypeptide-associated complex beta /NACB) is a member of the NAC-beta family of transcription factors. It is ubiquitously expressed and involved in the initiation of gene transcription by RNA polymerase. BTF3 also appears to impact estrogen receptor alpha (ER alpha ) stimulation of gene activation by binding to both ligand activated and non-ligand activated ER alpha. Human BTF3 is 206 amino acids (aa) in length and contains one potential phosphorylation site at Ser30, and a NACB domain (aa 82-147). There are multiple potential splice variants. Two variants show alternate start sites, one at Met45 (called BTF3b), and another at Met108.

Long Name

Basic Transcription Factor 3

Entrez Gene IDs

689 (Human); 218490 (Mouse); 498293 (Rat)

Alternate Names

basic transcription factor 3; BETA-NAC; BTF3; BTF3a; BTF3b; NACB; nascent-polypeptide-associated complex beta polypeptide; RNA polymerase B transcription factor 3; transcription factor BTF3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat BTF3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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