Home / Calcineurin A / Human/Mouse/Rat Calcineurin A Antibody

Human/Mouse/Rat Calcineurin A Antibody

Catalog #: MAB2839
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2839
MAB2839-SP
Detection of Human/Mouse/Rat Calcineurin A by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Calcineurin A Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Calcineurin A.
Source
Monoclonal Rat IgG1 Clone # 302202
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Bovine brain Calcineurin holoenzyme (A and B subunits)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Calcineurin A antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Calcineurin A by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat Calcineurin A Monoclonal Antibody (Catalog # MAB2839) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Calcineurin (Catalog # 3160-CA) (1 ng) was included. A specific band for Calcineurin A was detected at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calcineurin A

Calcineurin A, also known as PP2B and PPP3CA, is the 59 kDa catalytic subunit of the calcium/calmodulin-dependent protein phosphatase. When activated by calcium in the presence of the regulatory B subunit and calmodulin, Calcineurin A selectively removes phosphates from serine and threonine residues on target proteins. Although ubiquitously expressed, Calcineurin levels are highest in brain, where the phosphatase plays a role in the formation and retention of memories. Calcineurin activity is inhibited by the immunosuppressant drug cyclosporine A bound to cyclophilins.

Entrez Gene IDs
5530 (Human); 19055 (Mouse); 24674 (Rat)
Alternate Names
calcineurin A alpha; Calcineurin A; Calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA; CALNAcatalytic subunit, alpha isoform; CAM-PRP catalytic subunit; CNA; CNA1; CNA1CALNA1; EC 3.1.3.16; PP2B; PP3CA; PPP2B; PPP2BCCN1; PPP3CA; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha); protein phosphatase 3, catalytic subunit, alpha isozyme; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

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Citations for Human/Mouse/Rat Calcineurin A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6.
    Authors: Gruol DL, Puro A, Hao C et al.
    J Neuroimmunol.
  2. RCAN1.4 regulates VEGFR-2 internalisation, cell polarity and migration in human microvascular endothelial cells
    Authors: Ahmad F. Alghanem, Emma L. Wilkinson, Maxine S. Emmett, Mohammad A. Aljasir, Katherine Holmes, Beverley A. Rothermel et al.
    Angiogenesis
  3. Insulin dysfunction induces in vivo tau hyperphosphorylation through distinct mechanisms.
    Authors: Planel E, Tatebayashi Y, Miyasaka T, Liu L, Wang L, Herman M, Yu WH, Luchsinger JA, Wadzinski B, Duff KE, Takashima A
    J. Neurosci., 2007-12-12;27(50):13635-48.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis
    Authors: Ajay Mishra, Bénédicte Oulès, Angela Oliveira Pisco, Tony Ly, Kifayathullah Liakath-Ali, Gernot Walko et al.
    eLife

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