Human/Mouse/Rat Calcineurin B Antibody

Catalog #: AF1348
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1348
AF1348-SP

Detection of Human/Mouse/Rat Calcineurin B by Western Blot.

2 Images

Product Details

Citations (6)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Calcineurin B Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Calcineurin B in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Calcineurin B
Accession # P63098

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Simple Western

2.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human/Mouse/Rat Calcineurin B by Western Blot. Western blot shows lysates of mouse brain tissue, HT-29 human colon adenocarcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat Calcineurin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1348) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Calcineurin B at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

View Larger

Detection of Human Calcineurin B by Simple Western^TM. Simple Western lane view shows lysates of HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Calcineurin B at approximately 24 kDa (as indicated) using 2.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Calcineurin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1348). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calcineurin B

Calcineurin, also called Protein Phosphatase 2B, PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59,000 dalton catalytic A subunit and a 1,900 dalton regulatory B subunit that is activated by the binding of calcium ions and calmodulin (1). Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory (2). It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation (3). Complexes of the immunosuppressants cyclosporin and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity (4). Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy (5) and graft versus host disease in organ transplantation (6).

References

Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.
Zeng H. et al. (2001) Cell 107:617.
Okamura, H. et al. (2000) Molecular Cell 6:539.
Liu, J. et al. (1992) Biochemistry 31:3896.
Molkentin, J.D. (2000) Circulation Research 87:731.
Sanquer, S.A. et al. (2004) Transplantation 77:854.

Entrez Gene IDs

5534 (Human); 19058 (Mouse); 29748 (Rat)

Alternate Names

Calcineurin B; calcineurin subunit B type 1; CALNB1; CNA2; Cnb1; MCIP1; PP2B beta 1; PPP3R1; Protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3, regulatory subunit B, alpha; type I (19kDa)

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Calcineurin B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

Pin1 mediates A beta 42 -induced dendritic spine loss
Authors: Nancy R. Stallings, Melissa A. O’Neal, Jie Hu, Ege T. Kavalali, Ilya Bezprozvanny, James S. Malter
Science Signaling
Long-term running exercise alleviates cognitive dysfunction in APP/PSEN1 transgenic mice via enhancing brain lysosomal function
Authors: X Wang, YT Zhu, Y Zhu, YL Sun, J Huang, Z Li, Y Wang, JC Wu, ZH Qin, F Lin
Acta pharmacologica Sinica, 2021-07-16;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot
Combinatory FK506 and Minocycline Treatment Alleviates Prion-Induced Neurodegenerative Events via Caspase-Mediated MAPK-NRF2 Pathway
Authors: SZA Shah, D Zhao, G Taglialate, T Hussain, H Dong, N Sabir, MH Mangi, W Wu, M Lai, X Zhang, Y Duan, L Wang, X Zhou, L Yang
Int J Mol Sci, 2019-03-06;20(5):.
Species: Hamster
Sample Types: Tissue Homogenates
Applications: Western Blot
MicroRNA interactome analysis predicts post-transcriptional regulation of ADRB2 and PPP3R1 in the hypercholesterolemic myocardium
Authors: B Ágg, T Baranyai, A Makkos, B Vet?, N Faragó, Á Zvara, Z Giricz, DV Veres, P Csermely, T Arányi, LG Puskás, ZV Varga, P Ferdinandy
Sci Rep, 2018-07-04;8(1):10134.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
Phosphatase inhibition increases AQP2 accumulation in the rat IMCD apical plasma membrane
Authors: Janet D Klein
Am. J. Physiol. Renal Physiol., 2016-08-03;311(6):F1189-F1197.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
Regulation of RCAN1 protein activity by Dyrk1A protein-mediated phosphorylation.
Authors: Jung MS, Park JH, Ryu YS, Choi SH, Yoon SH, Kwen MY, Oh JY, Song WJ, Chung SH
J. Biol. Chem., 2011-09-30;286(46):40401-12.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot