Human/Mouse/Rat CBP Antibody

Catalog #: MAB2676
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2676
MAB2676-SP

Detection of Human, Mouse, and Rat CBP by Western Blot.

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All CBP Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat CBP Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat CBP.

Source

Monoclonal Mouse IgG_2B Clone # 257003

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CBP
Asn2141-Leu2442
Accession # Q92793

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

Immersion fixed HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat CBP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat CBP Monoclonal Antibody (Catalog # MAB2676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CBP at approximately 265 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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CBP in HeLa Human Cell Line. CBP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat CBP Monoclonal Antibody (Catalog # MAB2676) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CBP

The CBP (CREB-binding protein) is a histone acetyltransferase and transcriptional coactivator that regulates RNA polymerase II transcription. In addition to histone acetylation, CBP also acetylates various transcription factors, including the tumor suppressor protein p53, to enhance sequence-specific DNA binding.

Long Name

CREB-binding Protein

Entrez Gene IDs

1387 (Human); 12914 (Mouse); 54244 (Rat)

Alternate Names

CBP; CBPRTS; CREB binding protein; CREB-binding protein; CREBBP; EC 2.3.1.48; KAT3A; RSTS; RTS; Rubinstein-Taybi syndrome

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat CBP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Respiratory syncytial virus impairs macrophage IFN-alpha/beta- and IFN-gamma-stimulated transcription by distinct mechanisms.
Authors: Senft AP, Taylor RH, Lei W, Campbell SA, Tipper JL, Martinez MJ, Witt TL, Clay CC, Harrod KS
Am. J. Respir. Cell Mol. Biol., 2009-06-05;42(4):404-14.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Effect of cAMP on TGFbeta1-induced corneal keratocyte-myofibroblast transformation.
Authors: Xing D, Bonanno JA
Invest. Ophthalmol. Vis. Sci., 2008-10-20;50(2):626-33.
Species: Rabbit
Sample Types: Cell Lysates
Applications: Co-Immunoprecipitation