Home / A2B5 / Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody

Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB1416G
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1416G-100UG
Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Specificity
Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).
Source
Monoclonal Mouse IgM Clone # 105
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Embryonic chicken retinal cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Samples used for this experiment were NTera‑2 cells treated with retinoic acid and  beta TC-6 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of A2B5 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either treated with 10 μM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1416G). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of A2B5 in beta TC-6 cells by Flow Cytometry. beta TC-6 cells were stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1416G, filled histogram) or isotype control antibody (Catalog # IC015G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: A2B5

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).

References
  1. Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
  2. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
  3. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
  4. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.
Alternate Names
A2B5 marker; A2B5

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. PD-L1 positive astrocytes attenuate inflammatory functions of PD-1 positive microglia in models of autoimmune neuroinflammation
    Authors: Linnerbauer M, Beyer T, Nirschl L et al.
    Nat Commun
  2. PD-L1 positive astrocytes attenuate inflammatory functions of PD-1 positive microglia in models of autoimmune neuroinflammation
    Authors: Linnerbauer M, Beyer T, Nirschl L et al.
    Nat Commun
  3. Astrocyte-Derived Pleiotrophin Mitigates Late-Stage Autoimmune CNS Inflammation
    Authors: Linnerbauer M, L��lein L, Farrenkopf D et al.
    Frontiers in Immunology
  4. Gene Expression Analysis of Astrocyte and Microglia Endocannabinoid Signaling during Autoimmune Demyelination
    Authors: Á Moreno-Gar, A Bernal-Chi, T Colomer, A Rodríguez-, C Matute, S Mato
    Biomolecules, 2020-08-24;10(9):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Astrocyte-Derived Pleiotrophin Mitigates Late-Stage Autoimmune CNS Inflammation
    Authors: Linnerbauer M, L��lein L, Farrenkopf D et al.
    Frontiers in Immunology

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