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Human/Mouse/Rat/Chicken A2B5 Antibody

Catalog #: MAB1416
27 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1416
MAB1416-SP
Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry.
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Citations (27)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat/Chicken A2B5 Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Specificity
Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).
Source
Monoclonal Mouse IgM Clone # 105
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Embryonic chicken retinal cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of A2B5 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line either treated with 10 µM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry A2B5 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

A2B5 in NTera-2 Human Cell Line. A2B5 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line (differentiated with retinoic acid for 10 days) using Mouse Anti-Human/Mouse/Rat/ Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A2B5

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).

References
  1. Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
  2. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
  3. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
  4. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.
Alternate Names
A2B5 marker; A2B5

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Citations for Human/Mouse/Rat/Chicken A2B5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Repurposing the cardiac glycoside digoxin to stimulate myelin regeneration in chemically-induced and immune-mediated mouse models of multiple sclerosis
    Authors: Titus HE, Xu H, Robinson AP et al.
    Glia
  2. Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression
    Authors: Dou Ye, Suqing Qu, Yinxiang Yang, Zhaoyan Wang, Qian Wang, Weipeng Liu et al.
    iScience
  3. Differentiation of Spiral Ganglion-Derived Neural Stem Cells into Functional Synaptogenetic Neurons
    Authors: Xiaoyang Li, Alicia Aleardi, Jue Wang, Yang Zhou, Rodrigo Andrade, Zhengqing Hu
    Stem Cells and Development
  4. Human glial-restricted progenitors survive, proliferate, and preserve electrophysiological function in rats with focal inflammatory spinal cord demyelination
    Authors: Piotr Walczak, Angelo H. All, Nidhi Rumpal, Michael Gorelik, Heechul Kim, Anil Maybhate et al.
    Glia
  5. CD44 correlates with longevity and enhances basal ATF6 activity and ER stress resistance
    Authors: Takasugi, M;Ohtani, N;Takemura, K;Emmrich, S;Zakusilo, FT;Yoshida, Y;Kutsukake, N;Mariani, JN;Windrem, MS;Chandler-Militello, D;Goldman, SA;Satoh, J;Ito, S;Seluanov, A;Gorbunova, V;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Donor cell memory confers a metastable state of directly converted cells
    Authors: KP Kim, C Li, D Bunina, HW Jeong, J Ghelman, J Yoon, B Shin, H Park, DW Han, JB Zaugg, J Kim, T Kuhlmann, RH Adams, KM Noh, SA Goldman, HR Schöler
    Cell Stem Cell, 2021-04-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes
    Authors: M Yazdankhah, S Ghosh, P Shang, N Stepicheva, S Hose, H Liu, X Chamling, S Tian, MLG Sullivan, MJ Calderon, CS Fitting, J Weiss, A Jayagopal, JT Handa, JA Sahel, JS Zigler, PR Kinchingto, DJ Zack, D Sinha
    Autophagy, 2021-01-19;0(0):1-20.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  8. Regulation of human glioma cell migration, tumor growth, and stemness gene expression using a Lck targeted inhibitor
    Authors: JP Zepecki, KM Snyder, MM Moreno, E Fajardo, A Fiser, J Ness, A Sarkar, SA Toms, N Tapinos
    Oncogene, 2018-10-23;0(0):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: IHC
  9. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
    Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
    Cell Rep, 2017-04-04;19(1):20-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Protein-Induced Pluripotent Stem Cells Ameliorate Cognitive Dysfunction and Reduce A? Deposition in a Mouse Model of Alzheimer's Disease
    Authors: MY Cha, YW Kwon, HS Ahn, H Jeong, YY Lee, M Moon, SH Baik, DK Kim, H Song, EC Yi, D Hwang, HS Kim, I Mook-Jung
    Stem Cells Transl Med, 2016-08-15;6(1):293-305.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration.
    Authors: Moyon S, Dubessy A, Aigrot M, Trotter M, Huang J, Dauphinot L, Potier M, Kerninon C, Melik Parsadaniantz S, Franklin R, Lubetzki C
    J Neurosci, 2015-01-07;35(1):4-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Differentiation of human umbilical cord matrix mesenchymal stem cells into neural-like progenitor cells and maturation into an oligodendroglial-like lineage.
    Authors: Leite C, Silva N, Mendes S, Ribeiro A, de Faria J, Lourenco T, dos Santos F, Andrade P, Cardoso C, Vieira M, Paiva A, da Silva C, Cabral J, Relvas J, Graos M
    PLoS ONE, 2014-10-30;9(10):e111059.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  13. Characterization of oligodendroglial populations in mouse demyelinating disease using flow cytometry: clues for MS pathogenesis.
    Authors: Robinson A, Rodgers J, Goings G, Miller S
    PLoS ONE, 2014-09-23;9(9):e107649.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Generation of neuronal progenitor cells in response to tumors in the human brain.
    Authors: Macas J, Ku M, Nern C, Xu Y, Buhler H, Remke M, Synowitz M, Franz K, Seifert V, Plate K, Kettenmann H, Glass R, Momma S
    Stem Cells, 2014-01-01;32(1):244-57.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
    Authors: Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J
    Nat Biotechnol, 2013-04-14;31(5):426-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. In vivo generation of neural tumors from neoplastic pluripotent stem cells models early human pediatric brain tumor formation.
    Authors: Werbowetski-Ogilvie TE, Morrison LC, Fiebig-Comyn A, Bhatia M
    Stem Cells, 2012-03-01;30(3):392-404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  17. Disrupted-in-Schizophrenia-1 expression is regulated by beta-site amyloid precursor protein cleaving enzyme-1-neuregulin cascade.
    Authors: Seshadri S, Kamiya A, Yokota Y, Prikulis I, Kano S, Hayashi-Takagi A, Stanco A, Eom TY, Rao S, Ishizuka K, Wong P, Korth C, Anton ES, Sawa A
    Proc. Natl. Acad. Sci. U.S.A., 2010-03-08;107(12):5622-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Tim-2 is the receptor for H-ferritin on oligodendrocytes.
    Authors: Todorich B, Zhang X, Slagle-Webb B, Seaman WE, Connor JR
    J. Neurochem., 2008-11-05;107(6):1495-505.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  19. Musashi1 RNA-binding protein regulates oligodendrocyte lineage cell differentiation and survival.
    Authors: Dobson NR, Zhou YX, Flint NC, Armstrong RC
    Glia, 2008-02-01;56(3):318-30.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  20. AMPA-mediated excitotoxicity in oligodendrocytes: role for Na(+)-K(+)-Cl(-) co-transport and reversal of Na(+)/Ca(2+) exchanger.
    Authors: Chen H, Kintner DB, Jones M, Matsuda T, Baba A, Kiedrowski L, Sun D
    J. Neurochem., 2007-05-08;102(6):1783-95.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  21. Efficient induction of oligodendrocytes from human embryonic stem cells.
    Authors: Kang SM, Cho MS, Seo H, Yoon CJ, Oh SK, Choi YM, Kim DW
    Stem Cells, 2006-10-19;25(2):419-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Cyclopamine treatment of human embryonic stem cells followed by culture in human astrocyte medium promotes differentiation into nestin- and GFAP-expressing astrocytic lineage.
    Authors: Lee DS, Yu K, Rho JY, Lee E, Han JS, Koo DB, Cho YS, Lee KK, Han YM
    Life Sci., 2006-09-23;80(2):154-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
    Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
    Brain Res. Dev. Brain Res., 2004-09-17;152(2):159-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Excitatory amino acid induced oligodendrocyte cell death in vitro: receptor-dependent and -independent mechanisms.
    Authors: Rosin C, Bates TE, Skaper SD
    J. Neurochem., 2004-09-01;90(5):1173-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal.
    Authors: Tsai HH, Tessier-Lavigne M, Miller RH
    Development, 2003-05-01;130(10):2095-105.
    Species: Chicken, Rat
    Sample Types: Whole Cells
    Applications: ICC
  26. Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature Oligodendrocytes
    Authors: Ye D, Wang Q, Yang Y et al.
    Cellular and molecular neurobiology
  27. Identifying the functions of two biomarkers in human oligodendrocyte progenitor cell development
    Authors: Haipeng Zhou, Ying He, Zhaoyan Wang, Qian Wang, Caiyan Hu, Xiaohua Wang et al.
    Journal of Translational Medicine

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