Home / Oligodendrocyte Marker O1 / Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Antibody

Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Antibody

Catalog #: MAB1327
19 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1327
MAB1327-SP
Detection of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem Cells by Flow Cytometry.
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Product Details
Citations (19)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Specificity
Detects human, mouse, rat and chicken Oligodendrocyte Marker O1.
Source
Monoclonal Mouse IgM Clone # O1
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Bovine brain corpus callosum white matter
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Oligodendrocyte Marker O1 antibody in Rat Differentiated Cortical Stem Cells antibody by Flow Cytometry. View Larger

Detection of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem Cells by Flow Cytometry. Rat differentiated cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Monoclonal Antibody (Catalog # MAB1327, filled histogram) or mouse IgM isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Oligodendrocyte Marker O1 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Oligodendrocyte Marker O1 in Rat Cortical Stem Cells. Oligodendrocyte Marker O1 was detected in immersion fixed 7 day differentiated rat cortical stem cells using Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Monoclonal Antibody (Catalog # MAB1327) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). Specific staining was localized to oligodendrocytes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Oligodendrocyte Marker O1

Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O1 recognizes a glycolipid antigen that is expressed on the surface of late oligodendrocyte progenitors. It has been commonly used in conjunction with Oligodendrocyte Marker O4 antibody to define immature oligodendrocyte (1-6). Progenitors that are O4 antigen-positive and O1 antigen-negative have been shown to differentiate into O1 antigen-positive oligodendrocytes in vitro (7).

References
  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Sontheimer, H. et al. (1989) Neuron 2:1135.
  4. Hardy, R.J. and V.L. Friedrich Jr. (1996) Development 122:2059.
  5. Reynolds, R. and R. Hardy (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Cai, Z. et al. (2001) Brain Res. 898:126.
Alternate Names
Marker; Oligodendrocyte Marker O1

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Citations for Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Cerebrospinal fluid of progressive multiple sclerosis patients reduces differentiation and immune functions of oligodendrocyte progenitor cells
    Authors: Omri Zveik, Nina Fainstein, Ariel Rechtman, Nitzan Haham, Tal Ganz, Iris Lavon et al.
    Glia
  2. CD200 -dependent and -independent immune-modulatory functions of neural stem cells
    Authors: T Arad, N Fainstein, S Goldfarb, M Lachish, T Ganz, T Ben-Hur
    Stem Cell Research, 2021-10-07;56(0):102559.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  3. Donor cell memory confers a metastable state of directly converted cells
    Authors: KP Kim, C Li, D Bunina, HW Jeong, J Ghelman, J Yoon, B Shin, H Park, DW Han, JB Zaugg, J Kim, T Kuhlmann, RH Adams, KM Noh, SA Goldman, HR Schöler
    Cell Stem Cell, 2021-04-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Modelling Toxoplasma gondii infection in human cerebral organoids
    Authors: Hyang-Hee Seo, Hyo-Won Han, Sang-Eun Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang Cheol Kim et al.
    Emerging Microbes & Infections
  5. Lipid-specific IgMs induce antiviral responses in the CNS: implications for progressive multifocal leukoencephalopathy in multiple sclerosis
    Authors: L Hayden, T Semenoff, V Schultz, SF Merz, KJ Chapple, M Rodriguez, AE Warrington, X Shi, CS McKimmie, JM Edgar, K Thümmler, C Linington, M Pingen
    Acta Neuropathol Commun, 2020-08-13;8(1):135.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: Bioassay, ICC, Neutralization
  6. Susceptibility to erastin-induced ferroptosis decreases during maturation in a human oligodendrocyte cell line
    Authors: T Hoshino, H Yamakado, R Takahashi, SI Matsuzawa
    FEBS Open Bio, 2020-07-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. m6A mRNA Methylation Is Essential for Oligodendrocyte Maturation and CNS Myelination
    Authors: Huan Xu, Yulia Dzhashiashvili, Ankeeta Shah, Rejani B. Kunjamma, Yi-lan Weng, Benayahu Elbaz et al.
    Neuron
  8. Oligodendrocytes regulate presynaptic properties and neurotransmission through BDNF signaling in the mouse brainstem
    Authors: Miae Jang, Elizabeth Gould, Jie Xu, Eun Jung Kim, Jun Hee Kim
    eLife
  9. Differentiation and maturation of oligodendrocytes in human three-dimensional neural cultures
    Authors: RM Marton, Y Miura, SA Sloan, Q Li, O Revah, RJ Levy, JR Huguenard, SP Pa?ca
    Nat. Neurosci., 2019-01-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon
    Authors: S Gurung, N Reuter, A Preno, J Dubaut, H Nadeau, K Hyatt, K Singleton, A Martin, WT Parks, JF Papin, DA Myers
    PLoS Pathog., 2019-01-18;15(1):e1007507.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Oligodendrocyte differentiation of induced pluripotent stem cells derived from subjects with schizophrenias implicate abnormalities in development
    Authors: Donna L. McPhie, Ralda Nehme, Caitlin Ravichandran, Suzann M. Babb, Sulagna Dia Ghosh, Alexandra Staskus et al.
    Translational Psychiatry
  12. N-Phenylquinazolin-2-amine Yhhu4952 as a novel promotor for oligodendrocyte differentiation and myelination
    Authors: X Yu, G Cheng, L Zhang, Y Zhang, Q Wang, M Zhao, L Zeng, Y Hu, L Feng
    Sci Rep, 2018-09-19;8(1):14040.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Chondroitin sulfate proteoglycans inhibit the migration and differentiation of oligodendrocyte precursor cells and its counteractive interaction with laminin
    Authors: Yi Sun, Yingping Deng, Mili Xiao, Lan Hu, Zhihua Li, Chao Chen
    International Journal of Molecular Medicine
  14. A three-dimensional model of the human blood-brain barrier to analyse the transport of nanoparticles and astrocyte/endothelial interactions
    Authors: Peddagangannagari Sreekanthreddy, Radka Gromnicova, Heather Davies, James Phillips, Ignacio A. Romero, David Male
    F1000Research
  15. Characterization of oligodendroglial populations in mouse demyelinating disease using flow cytometry: clues for MS pathogenesis.
    Authors: Robinson A, Rodgers J, Goings G, Miller S
    PLoS ONE, 2014-09-23;9(9):e107649.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Uteroplacental insufficiency alters rat hippocampal cellular phenotype in conjunction with ErbB receptor expression
    Authors: Camille Fung, Xingrao Ke, Ashley S. Brown, Xing Yu, Robert A. McKnight, Robert H. Lane
    Pediatric Research
  17. Curcumin protects pre-oligodendrocytes from activated microglia in vitro and in vivo.
    Authors: He LF, Chen HJ, Qian LH, Chen GY, Buzby JS
    Brain Res., 2010-04-24;1339(0):60-9.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  18. Efficient induction of oligodendrocytes from human embryonic stem cells.
    Authors: Kang SM, Cho MS, Seo H, Yoon CJ, Oh SK, Choi YM, Kim DW
    Stem Cells, 2006-10-19;25(2):419-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Excitatory amino acid induced oligodendrocyte cell death in vitro: receptor-dependent and -independent mechanisms.
    Authors: Rosin C, Bates TE, Skaper SD
    J. Neurochem., 2004-09-01;90(5):1173-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Antibody
By Anonymous on 01/25/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: IPS2 induced pluripotent stem cells Species: Mouse
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Antibody MAB1327