Human/Mouse/Rat CIB1 Antibody

Catalog #: AF7557
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7557
AF7557-SP

Detection of Human, Mouse, and Rat CIB1 by Western Blot.

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All CIB1 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat CIB1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat CIB1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CIB1
Gly2-Leu191
Accession # Q99828

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat CIB1 by Western Blot. Western blot shows lysates of human spleen tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat CIB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CIB1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CIB1

CIB1 (Calcium and Integrin-Binding protein 1; also calmyrin, KIP and CIBP) is a 22-23 kDa, Ca⁺⁺-binding member of the CIB family of proteins. It is widely expressed, being found in neurons, platelets/megakaryocytes, skeletal muscle myocytes and lymphocytes. CIB1 is associated with the cytosolic side of the plasma membrane, and has multiple binding partners, including InsP₃R, GPIIb/ alpha IIb, presenilin 2, and NBR1 plus FEZ. When CIB1 binds InsP₃R, this ER-embedded receptor is both activated, and later desensitized to subsequent ligand binding. Relative to GPIIb, CIB1 interaction with the alpha IIb beta ₃ integrin on platelets following thrombin exposure appears to inhibit integrin activation, thus providing a tight control on subsequent platelet binding to fibrinogen. Human CIB1 is 191 amino acids (aa) in length. It contains a utilized myristoylation site at Gly2, followed by two EF-hand domains (aa 103-183). CIB1 is suggested to act as a monomer. There is at least one potential isoform variant that shows a 40 aa insertion after Lys29. Full-length human CIB1 (aa 1-191) shares 94% aa sequence identity with mouse CIB1.

Long Name

Calcium and Integrin Binding 1

Entrez Gene IDs

10519 (Human); 23991 (Mouse); 81823 (Rat)

Alternate Names

calcium and integrin binding 1 (calmyrin); Calcium- and integrin-binding protein; Calmyrin; CIB1; CIBcalcium and integrin binding protein; CIBP; DNA-dependent protein kinase interacting protein; DNA-PKcs-interacting protein; Kinase-interacting protein; KIP; KIP1; KIPcalcium and integrin binding, protein kinase interacting protein; PRKDCIP; SIP2-28; SIP2-28calcium and integrin-binding protein 1; Snk interacting protein 2-28; SNK-interacting protein 2-28

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human/Mouse/Rat CIB1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Expression of a TMC6-TMC8-CIB1 heterotrimeric complex in lymphocytes is regulated by each of the components
Authors: CJ Wu, X Li, CL Sommers, K Kurima, S Huh, G Bugos, L Dong, W Li, AJ Griffith, LE Samelson
J. Biol. Chem., 2020-09-11;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot