Human/Mouse/Rat DARPP-32 Antibody

Catalog #: AF6259
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6259
AF6259-SP

Detection of Human, Mouse, and Rat DARPP‑32 by Western Blot.

4 Images

All DARPP-32 Antibodies

Product Details

Citations (6)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat DARPP-32 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat DARPP‑32 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DARPP‑32
Arg51-Ala204
Accession # Q9UD71

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat DARPP‑32 by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DARPP-32 at approximately 32 - 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

DARPP‑32 in Human Brain. DARPP-32 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

View Larger

DARPP‑32 in Mouse Brain. DARPP-32 was detected in perfusion fixed frozen sections of mouse brain (caudate putamen) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

View Larger

DARPP‑32 in Rat Brain. DARPP-32 was detected in perfusion fixed frozen sections of rat brain (globus pallidus) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DARPP-32

Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kDa protein that anomalously migrates at about 32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP‑32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.

Long Name

Dopamine and cAMP-regulated Phosphoprotein

Entrez Gene IDs

84152 (Human); 19049 (Mouse); 360616 (Rat)

Alternate Names

DARPP32; DARPP-32; PPP1R1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B; regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat DARPP-32 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

Cholinergic neurons constitutively engage the ISR for dopamine modulation and skill learning in mice
Authors: Helseth AR, Hernandez-Martinez R, Hall VL et al.
Science (New York, N.Y.)
Interleukin-9 protects from microglia- and TNF-mediated synaptotoxicity in experimental multiple sclerosis
Authors: Guadalupi, L;Vanni, V;Balletta, S;Caioli, S;De Vito, F;Fresegna, D;Sanna, K;Nencini, M;Donninelli, G;Volpe, E;Mariani, F;Battistini, L;Stampanoni Bassi, M;Gilio, L;Bruno, A;Dolcetti, E;Buttari, F;Mandolesi, G;Centonze, D;Musella, A;
Journal of neuroinflammation
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry
Dopaminergic Input Regulates the Sensitivity of Indirect Pathway Striatal Spiny Neurons to Brain-Derived Neurotrophic Factor
Authors: Ayon-Olivas, M;Wolf, D;Andreska, T;Granado, N;Lüningschrör, P;Ip, CW;Moratalla, R;Sendtner, M;
Biology
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Alpha‐Synuclein is Involved in DYT1 Dystonia Striatal Synaptic Dysfunction
Authors: Giulia Ponterio, Gaia Faustini, Ilham El Atiallah, Giuseppe Sciamanna, Maria Meringolo, Annalisa Tassone et al.
Movement Disorders
CRISPR-Cas9-Mediated Genome Editing Increases Lifespan and Improves Motor Deficits in a Huntington’s Disease Mouse Model
Authors: Freja K. Ekman, David S. Ojala, Maroof M. Adil, Paola A. Lopez, David V. Schaffer, Thomas Gaj
Molecular Therapy - Nucleic Acids
Early structural and functional plasticity alterations in a susceptibility period of DYT1 dystonia mouse striatum
Authors: M Maltese, J Stanic, A Tassone, G Sciamanna, G Ponterio, V Vanni, G Martella, P Imbriani, P Bonsi, NB Mercuri, F Gardoni, A Pisani
Elife, 2018-03-05;7(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr