Human/Mouse/Rat eEF-2 Antibody

Catalog #: AF3806 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3806
AF3806-SP

Detection of Human/Mouse/Rat eEF‑2 by Western Blot.

1 Image

All eEF-2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat eEF-2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat eEF-2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human eEF-2
Val300-Ser500
Accession # Q6PK56

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human/Mouse/Rat eEF‑2 by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat eEF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3806) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eEF-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eEF-2

The eukaryotic translation elongation factor-2 (eEF-2) is a GTP-binding protein that is required for the translocation step from the A site to the P site of the ribosome. Phosphorylation of eEF-2 by the eEF-2 kinase causes complete inhibition of its activity.

Long Name

Eukaryotic Translation Elongation Factor 2

Entrez Gene IDs

1938 (Human); 13629 (Mouse); 29565 (Rat)

Alternate Names

eEF2; eEF-2; EF-2; EF2EEF-2; elongation factor 2; eukaryotic translation elongation factor 2; polypeptidyl-tRNA translocase