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Human/Mouse/Rat EG-VEGF/PK1 Antibody

Catalog #: MAB2100
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2100
MAB2100-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat EG-VEGF/PK1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat EG-VEGF in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human EG-VEGF and recombinant mouse EG-VEGF is observed.
Source
Monoclonal Mouse IgG2B Clone # 239727
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat EG-VEGF
Ala20-Phe105 (predicted)
Accession # Q8R414
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL

Recombinant Human EG-VEGF/PK1 (Catalog # 1209-EV)
Recombinant Mouse EG-VEGF/PK1 (Catalog # 2464-EV)
Recombinant Rat EG-VEGF/PK1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EG-VEGF/PK1

EG-VEGF, a member of the prokineticin family of secreted proteins, is expressed in multiple tissues including the gastrointestinal tract, testis, ovary, placenta and adrenal glands. It binds to the G protein-coupled receptors, EG-VEGF/PK1-R1 and EG-VEGF/PK2-R2, leading to stimulation of smooth muscle contraction and tissue‑specific angiogenesis.

Long Name
Endocrine Gland-derived Vascular Endothelial Growth Factor
Entrez Gene IDs
84432 (Human); 246691 (Mouse); 192205 (Rat)
Alternate Names
EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1

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Citation for Human/Mouse/Rat EG-VEGF/PK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The prokineticin receptor-1 (GPR73) promotes cardiomyocyte survival and angiogenesis.
    Authors: Urayama K, Guilini C, Messaddeq N, Hu K, Steenman M, Kurose H, Ert G, Nebigil CG
    FASEB J., 2007-04-18;21(11):2980-93.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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