Human/Mouse/Rat EMP/MAEA Antibody Summary
Met12-Ser66
Accession # Q7L5Y9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat EMP/MAEA by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7288) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMP/MAEA at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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EMP/MAEA in Jurkat Human Cell Line. EMP/MAEA was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Sheep Anti-Human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7288) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Mouse EMP/MAEA by Western Blot V5-HA-tagged RanBP9 maintains its ability to interact with known members of the CTLH complex and Nucleolin. RanBP9 WT and TT immortalized Mouse Embryonic Fibroblasts (MEFs) were cultured in standard conditions and protein lysates were obtained. Resin conjugated with alpha HA antibodies was used to immunoprecipitate RanBP9-TT protein. IPed fractions and 5% of input were run on gels to generate 5 different membranes that were probed with the indicated antibodies by WB. Vinculin is used as loading control. Shown results are representative of two independent experiments (biological replicates). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32346083), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EMP/MAEA
EMP (erythroblast macrophage protein), also called MAEA (macrophage-erythroblast attacher), is a 36-45 kDa membrane-associated protein in macrophages and erythroblasts within erythroblastic islands. EMP is essential to the process of nuclear extrusion in the transition of erythroblasts to reticulocytes. Within the region used as an immunogen, human EMP shares 98% amino acid (aa) sequence identity with mouse and rat EMP. Four potential isoforms of the canonical 396 aa form encode 360, 355, 328 and 245 aa, with insertions and deletions occurring after aa 150.
Product Datasheets
Citations for Human/Mouse/Rat EMP/MAEA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
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The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation
Authors: F Lampert, D Stafa, A Goga, MV Soste, S Gilberto, N Olieric, P Picotti, M Stoffel, M Peter
Elife, 2018-06-18;7(0):.
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GID E3 ligase supramolecular chelate assembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzyme
Authors: Sherpa D, Chrustowicz J, Qiao S et al.
Molecular cell
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Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth
Authors: Simwela, NV;Johnston, L;Pavinski Bitar, P;Jaecklein, E;Altier, C;Sassetti, CM;Russell, DG;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The CTLH Ubiquitin Ligase Substrates ZMYND19 and MKLN1 Negatively Regulate mTORC1 at the Lysosomal Membrane
Authors: Wang, Y;Guo, R;Piedras, BI;Tang, HY;Asara, JM;Tempera, I;Lieberman, PM;Gewurz, BE;
Research square
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin
Authors: SHA Soliman, AE Stark, ML Gardner, SW Harshman, CC Breece, F Amari, A Orlacchio, M Chen, A Tessari, JA Martin, R Visone, MA Freitas, KMD La Perle, D Palmieri, V Coppola
Sci Rep, 2020-04-28;10(1):7138.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation
Authors: MER Maitland, G Onea, CA Chiasson, X Wang, J Ma, SE Moor, KR Barber, GA Lajoie, GS Shaw, C Schild-Pou
Sci Rep, 2019-07-08;9(1):9864.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
The human GID complex engages two independent modules for substrate recruitment
Authors: Weaam I Mohamed, Sophia L Park, Julius Rabl, Alexander Leitner, Daniel Boehringer, Matthias Peter
EMBO reports
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