Human/Mouse/Rat FABP1/L-FABP Antibody

Catalog # Availability Size / Price Qty
MAB29641
MAB29641-SP
Detection of Human FABP1/L‑FABP by Western Blot.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse/Rat FABP1/L-FABP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human FABP1/L-FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with rhFABP2, -3, -4, -5, -6, -7, or rmFABP9 is observed.
Source
Monoclonal Mouse IgG1 Clone # 328605
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FABP1/L-FABP
Met1-Ile127
Accession # P07148
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
5-10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FABP1/L‑FABP antibody by Western Blot. View Larger

Detection of Human FABP1/L‑FABP by Western Blot. Western blot shows lysates of human liver tissue, human colon tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/ L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human, Mouse, and Rat FABP1/L-FABP antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/ L-FABP Monoclonal Antibody (Catalog # MAB29641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human FABP1/L-FABP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human FABP1/L‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 14 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/ L‑FABP Monoclonal Antibody (Catalog # MAB29641). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Human and Mouse FABP1/L-FABP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse FABP1/L‑FABP by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 14-16 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB29641). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP1/L-FABP

FABP1, also known as liver FABP (L-FABP), Z-protein and squalene-and sterol-carrier protein (SCP) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.

Long Name
Fatty Acid-Binding Protein 1
Entrez Gene IDs
2168 (Human); 14080 (Mouse); 24360 (Rat)
Alternate Names
FABP1; FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; LFABP; L-FABP; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein

Product Datasheets

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Citation for Human/Mouse/Rat FABP1/L-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Serum Liver Fatty Acid Binding Protein Levels Correlate Positively with Obesity and Insulin Resistance in Chinese Young Adults
    Authors: Juan Shi, Yifei Zhang, Weiqiong Gu, Bin Cui, Min Xu, Qun Yan et al.
    PLoS ONE

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Reviews for Human/Mouse/Rat FABP1/L-FABP Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Human/Mouse/Rat FABP1/L-FABP Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat FABP1/L-FABP Antibody
By Anonymous on 02/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human/Mouse/Rat FABP1/L-FABP Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human