Human/Mouse/Rat FBPase 1 Antibody

Catalog #: AF7285 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7285
AF7285-SP

Detection of Human, Mouse, and Rat FBPase 1 by Western Blot.

2 Images

All FBPase 1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat FBPase 1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, rat FBPase 1 in Western blots and detects human FBPase 1 in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FBPase 1
Ala2-Gln338 (Arg218Lys)
Accession # P09467

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat FBPase 1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat FBPase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7285) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FBPase 1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

FBPase 1 in Human Liver Cancer Tissue. FBPase 1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Sheep Anti-Human/Mouse/Rat FBPase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7285) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FBPase 1

FBPase 1 (Fructose 1,6 bisphosphatase 1 [Fru-1,6-P2ase]; also FBP-1 phosphohydrolase 1) is a 35-37 kDa member of the FBPase Class I family of enzymes. It is widely expressed, being found in cells diverse as monocytes, hepatocytes, pancreatic beta -cells and striated (skeletal plus cardiac) muscle cells. FBPase 1 converts Fru-1,6-P2 into Fru-6-P, a molecule that is subsequently used to generate glucose. Human FBPase 1 is 338 amino acids (aa) in length. It contains one catalytic region (aa 18-332) and an NLS between aa 203-208. FBPase 1 exhibits divalent cation dependency, and exists as a homotetramer within the cell. Full-length human FBPase 1 shares 85% aa sequence identity with mouse FBPase 1.

Long Name

Fructose-1,6-bisphosphatase 1

Entrez Gene IDs

2203 (Human); 14121 (Mouse); 24362 (Rat)

Alternate Names

D-fructose-1,6-bisphosphate 1-phosphohydrolase 1; EC 3.1.3; EC 3.1.3.11; FBP; FBP1; FBPase 1; fructose-1,6-bisphosphatase 1; fructose-bisphosphatase 1; growth-inhibiting protein 17