Home / FKBP12 / Human/Mouse/Rat FKBP12 Antibody

Human/Mouse/Rat FKBP12 Antibody

Catalog #: MAB3777
4 Citations Datasheet / COA / SDS
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MAB3777
MAB3777-SP
Detection of Human/Mouse/Rat FKBP12 by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat FKBP12 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat FKBP12 in Western blots. In Western blots, no cross-reactivity with other FKBP family members is observed.
Source
Monoclonal Rat IgG2B Clone # 422513
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FKBP12
Gly2-Glu108
Accession # P62942
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat FKBP12 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat FKBP12 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat FKBP12 Monoclonal Antibody (Catalog # MAB3777) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human FKBP12 (Catalog # 3777-FK), recombinant human FKBP12.6, and recombinant human FKBP13 (5 ng/lane) were included. A specific band for FKBP12 was detected at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP12

FK506 binding protein, 12 kilodalton molecular weight (FKBP12), also called FKBP1, was originally characterized as a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. Proline isomerase activity was demonstrated but not used for quality control. The macrolide immunosuppressants FK506 (Tacrolimus) and rapamycin bind to FKBP12 with high affinity, while the structurally related compound cyclosporine binds with a much lower affinity (1). The binding of these drugs causes FKBP12 to become a potent inhibitor of calcineurin phosphatase activity (2) and TOR kinase activity (3). The inhibition of protein phosphatase activity is highly selective for calcineurin (2), making the FK506/FKBP12 complex a useful tool in the study of this enzyme. Knockout mice lacking FKBP12 are morphologically normal, but develop cardiomyopathies that may be related to dysregulation of ryanodyne receptors (4).

References
  1. Hamilton, G.S. and J.P. Steiner (1998) J. Med. Chem. 41:5119.
  2. Liu, J. et al. (1992) Biochemistry 31:3896.
  3. Toral-Barza, L. et al. (2005) Biochem. Biophys. Res. Comm. 332:304.
  4. Hamilton, S.L and M.M. Matzuk (1998) Nature 391:489.
Long Name
12 kDa FK506 Binding Protein
Entrez Gene IDs
2280 (Human); 14225 (Mouse); 25639 (Rat)
Alternate Names
12 kDa FKBP; EC 5.2.1.8; FK506 binding protein 1A, 12kDa; FK506-binding protein 1A; FKBP1; FKBP12; FKBP12C; FKBP12FKBP12-Exip3; FKBP-12T-cell, 12-kD; FKBP1a; FKBP-1A; peptidyl-prolyl cis-trans isomerase FKBP1A; PKC12; PPIase FKBP1A; PPIASE; protein kinase C inhibitor 2; rotamase

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Citations for Human/Mouse/Rat FKBP12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Chaperoning of specific tau structure by immunophilin FKBP12 regulates the neuronal resilience to extracellular stress
    Authors: L Jiang, P Chakrabort, L Zhang, M Wong, SE Hill, CJ Webber, J Libera, LJ Blair, B Wolozin, M Zweckstett
    Science Advances, 2023-02-01;9(5):eadd9789.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms
    Authors: C Grohmann, CM Magtoto, JR Walker, NK Chua, A Gabrielyan, M Hall, SA Cobbold, S Mieruszyns, M Brzozowski, DS Simpson, H Dong, B Dorizzi, AV Jacobsen, E Morrish, N Silke, JM Murphy, JK Heath, A Testa, C Maniaci, A Ciulli, G Lessene, J Silke, R Feltham
    Nature Communications, 2022-04-19;13(1):2073.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. An inducible, ligand-independent receptor activator of NF-kappaB gene to control osteoclast differentiation from monocytic precursors.
    Authors: Rementer C, Wu M, Buranaphatthana W, Yang H, Scatena M, Giachelli C
    PLoS ONE, 2013-12-27;8(12):e84465.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Designing Calcium Release Channel Inhibitors with Enhanced Electron Donor Properties: Stabilizing the Closed State of Ryanodine Receptor Type 1
    Authors: Yanping Ye, Daniel Yaeger, Laura J. Owen, Jorge O. Escobedo, Jialu Wang, Jeffrey D. Singer et al.
    Molecular Pharmacology

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