Human/Mouse/Rat FKBP12.6 Biotinylated Antibody

Catalog #: BAF4174 Datasheet / COA / SDS

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BAF4174

Detection of Human FKBP12.6 by Western Blot.

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Human/Mouse/Rat FKBP12.6 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat FKBP12.6 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FKBP12.6
Met1-Glu108
Accession # P68106

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FKBP12.6 by Western Blot. Western blot shows lysates of human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FKBP12.6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4174) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FKBP12.6 at approximately 12.6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP12.6

FK506 binding protein, 12.6 kDa molecular weight (FKBP12.6), also called FKBP1B, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑ proline residues critical for proper folding of proteins. The immunosuppressant FK506 is a potent inhibitor of FKBP12.6. FKBP12.6 is localized in the sarcoplasmic reticulum associated with the Ryanodyne receptor RYR2. PKA-induced phosphorylation of RYR2 causes FKBP12.6 dissociation. In heart failure, RYR2 is hyperphosphorylated and has low levels of FKBP12.6 bound to it. Male FKBP12.6 knockout mice have cardiac hypertrophy and lethal exercise arrhythmias.

Long Name

12.6 kDa FK506 Binding Protein

Entrez Gene IDs

2281 (Human)

Alternate Names

12.6 kDa FK506-binding protein; calstabin 2,12.6 kDa FKBP; EC 5.2.1.8; FK506 binding protein 1B, 12.6 kDa; FK506-binding protein 12.6; FK506-binding protein 1B (12.6 kD); FK506-binding protein 1B; FKBP12.6; FKBP-12.6; FKBP12.6h-FKBP-12; FKBP1B; FKBP-1B; FKBP1L; FKBP9; Immunophilin FKBP12.6; OTK4; OTK4PPIase FKBP1B; peptidyl-prolyl cis-trans isomerase FKBP1B; PKBP1L; PPIase; rotamase