Human/Mouse/Rat FKBP25 Antibody

Catalog #: MAB3955 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3955
MAB3955-SP

Detection of Human FKBP25 by Western Blot.

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Human/Mouse/Rat FKBP25 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat FKBP25 in Western blots. In Western blots, this antibody does not cross-react with recombinant human FKBP12, FKBP12.6, FKBP13, FKBP38, FKBP51, or FKBP52.

Source

Monoclonal Rat IgG_2B Clone # 423325

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FKBP25
Ala2-Asp224
Accession # Q00688

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FKBP25 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP25 Monoclonal Antibody (Catalog # MAB3955) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP25 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP25

FK506 binding protein, 25 kDa molecular weight (FKBP25), also called FKBP3, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressant Rapamycin is a potent inhibitor of FKBP25. FKBP25 is localized in the nucleus, where it alters the activity of histone deacetylases and transcription factors. It is found in many tissues, including brain, thymus, and spleen.

Long Name

25 kDa FK506 Binding Protein

Entrez Gene IDs

2287 (Human); 30795 (Mouse); 299104 (Rat)

Alternate Names

EC 5.2.1.8,25 kDa FK506-binding protein; FK506 binding protein 3, 25kDa; FK506-binding protein 3 (25kD); FK506-binding protein 3; FKBP25; FKBP25,25 kDa FKBP; FKBP3; FKBP-3; Immunophilin FKBP25; peptidyl-prolyl cis-trans isomerase FKBP3; PPIase FKBP3; PPIase; rapamycin binding protein; Rapamycin-selective 25 kDa immunophilin; rotamase; T-cell