Human/Mouse/Rat FKBP51 Antibody

Catalog #: MAB4094
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4094
MAB4094-SP

Detection of Human and Mouse FKBP51 by Western Blot.

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All FKBP51 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat FKBP51 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat FKBP51 in Western blots.

Source

Monoclonal Rat IgG_2B Clone # 425314

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FKBP51
Met1-Val457
Accession # Q13451

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse FKBP51 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP51 Monoclonal Antibody (Catalog # MAB4094) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP51 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP51

FK506 binding protein (FKBP51) is a 51 kDa peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans- proline residues critical for proper folding of proteins. The macrolide immunosuppressants FK506 and Rapamycin are FKBP51 inhibitors. FKBP51 levels are induced by glucocorticoids, and it associates with HSP90 complexes that are critical for the proper folding of steroid receptors. Single nucleotide polymorphisms in FKBP51 have been associated with major depression and hyper-responsiveness to antidepressants.

Long Name

51 kDa FK506 Binding Protein

Entrez Gene IDs

2289 (Human); 14229 (Mouse); 361810 (Rat)

Alternate Names

51 kDa FKBP; 54 kDa progesterone receptor-associated immunophilin; AIG6,51 kDa FK506-binding protein; Androgen-regulated protein 6; Dit1; FF1 antigen; FK506 binding protein 5; FK506-binding protein 5; FKBP5; FKBP-5; FKBP51; FKBP-51; FKBP51PPIase FKBP5; FKBP54; FKBP54EC 5.2.1.8; HSP90-binding immunophilin; MGC111006; p54; P54,51 kDa FK506-binding protein 5; peptidyl-prolyl cis-trans isomerase FKBP5; peptidylprolyl cis-trans isomerase; PPIase; Ptg-10; rotamase; T-cell FK506-binding protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat FKBP51 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Molecular Pathways Leading to Induction of Cell Death and Anti-Proliferative Properties by Tacrolimus and mTOR Inhibitors in Liver Cancer Cells
Authors: E Navarro-Vi, P de la Cruz, L Contreras, R González, M Negrete, MA Rodríguez-, LM Marín-Góme, JM Álamo-Mart, A Calvo, MA Gómez-Brav, J de la Cruz, J Padillo, J Muntané
Cell. Physiol. Biochem., 2020-05-06;54(3):457-473.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Sequencing the serotonergic neuron translatome reveals a new role for Fkbp5 in stress
Authors: AJ Lesiak, K Coffey, JH Cohen, KJ Liang, C Chavkin, JF Neumaier
Mol. Psychiatry, 2020-05-04;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Neuropsin cleaves EphB2 in the amygdala to control anxiety.
Authors: Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R
Nature, 2011-04-20;473(7347):372-5.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr