Human/Mouse/Rat FRS2 Antibody

Catalog #: MAB4069
13 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4069
MAB4069-SP

Detection of Human/Mouse/Rat FRS2 by Western Blot.

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Product Details

Citations (13)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat FRS2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat FRS2 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 462910

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FRS2
Asn121-Asn449
Accession # Q8WU20

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat FRS2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FRS2 Monoclonal Antibody (Catalog # MAB4069) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRS2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FRS2

FRS2 (FGF R substrate 2; also known as SNT and FRS2 alpha ) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF R and other RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain (aa 13‑115) that interacts with FGF and NGF receptors, and a C-terminal Tyr-rich region (aa 196‑471) that serves as a docking site for GRB‑2 and SHP‑2.

Long Name

Fibroblast Growth Factor Receptor Substrate 2

Entrez Gene IDs

10818 (Human); 327826 (Mouse)

Alternate Names

FGFR signalling adaptor; FGFR-signaling adaptor SNT; fibroblast growth factor receptor substrate 2; FRS2; FRS2A; FRS2alpha; SNT; SNT1; SNT-1FGFR substrate 2; Suc1-associated neurotrophic factor target 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat FRS2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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FGF19 functions as autocrine growth factor for hepatoblastoma
Authors: David J. Elzi, Meihua Song, Barron Blackman, Susan T. Weintraub, Dolores López-Terrada, Yidong Chen et al.
Genes & Cancer
Tackling FGFR3-driven bladder cancer with a promising synergistic FGFR/HDAC targeted therapy
Authors: Zechen Wang, Viswanathan Muthusamy, Daniel P. Petrylak, Karen S. Anderson
npj Precision Oncology
Inhibition of FGF receptor blocks adaptive resistance to RET inhibition in CCDC6-RET–rearranged thyroid cancer
Authors: Renuka Raman, Jacques A. Villefranc, Timothy M. Ullmann, Jessica Thiesmeyer, Viviana Anelli, Jun Yao et al.
Journal of Experimental Medicine
Distinct phenotypic consequences of cholangiocarcinoma-associated FGFR2 alterations depend on biliary epithelial maturity
Authors: O'Loughlin, E;Zhang, Y;Chiasson-MacKenzie, C;Dave, P;Rheinbay, E;Stott, S;McClatchey, AI;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
RLY-4008, the first highly selective FGFR2 inhibitor with activity across FGFR2 alterations and resistance mutations
Authors: Subbiah, V;Sahai, V;Maglic, D;Bruderek, K;Toure, BB;Zhao, S;Valverde, R;O'Hearn, PJ;Moustakas, DT;Schonherr, H;Gerami-Moayed, N;Taylor, AM;Hudson, BM;Houde, DJ;Pal, D;Foster, L;Gunaydin, H;Ayaz, P;Sharon, DA;Goyal, L;Schram, AM;Kamath, S;Sherwin, CA;Schmidt-Kittler, O;Jen, KY;Ricard, F;Wolf, BB;Shaw, DE;Bergstrom, DA;Watters, J;Casaletto, JB;
Cancer discovery
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Fibroblast growth factor signalling influences homologous recombination-mediated DNA damage repair to promote drug resistance in ovarian cancer
Authors: HA Nicholson, L Sawers, RG Clarke, KJ Hiom, MJ Ferguson, G Smith
British Journal of Cancer, 2022-07-01;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Whole-genome sequencing identifies ADGRG6 enhancer mutations and FRS2 duplications as angiogenesis-related drivers in bladder cancer
Authors: S Wu, T Ou, N Xing, J Lu, S Wan, C Wang, X Zhang, F Yang, Y Huang, Z Cai
Nat Commun, 2019-02-12;10(1):720.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Bradykinin B2 Receptor Contributes to Inflammatory Responses in Human Endothelial Cells by the Transactivation of the Fibroblast Growth Factor Receptor FGFR-1
Authors: E Terzuoli, F Corti, G Nannelli, A Giachetti, S Donnini, M Ziche
Int J Mol Sci, 2018-09-06;19(9):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Gambogenic acid inhibits fibroblast growth factor receptor signaling pathway in erlotinib-resistant non-small-cell lung cancer and suppresses patient-derived xenograft growth
Authors: L Xu, X Meng, N Xu, W Fu, H Tan, L Zhang, Q Zhou, J Qian, S Tu, X Li, Y Lao, H Xu
Cell Death Dis, 2018-02-15;9(3):262.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
Kidney Int, 2014-01-08;85(6):1340-50.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

Phosphorylation and lipid raft association of fibroblast growth factor receptor-2 in oligodendrocytes.
Authors: Bryant MR, Marta CB, Kim FS, Bansal R
Glia, 2009-07-01;57(9):935-46.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Preclinical characterization of bemarituzumab, an anti-FGFR2b antibody for the treatment of cancer
Authors: Hong Xiang, Abigael G. Chan, Ago Ahene, David I. Bellovin, Rong Deng, Amy W. Hsu et al.
mAbs

circKCNN2 suppresses the recurrence of hepatocellular carcinoma at least partially via regulating miR‐520c‐3p/methyl‐DNA‐binding domain protein 2 axis
Authors: Donghong Liu, Wenbin Liu, Xi Chen, Jianhua Yin, Longteng Ma, Mei Liu et al.
Clinical and Translational Medicine