Human/Mouse/Rat Gelsolin/GSN Antibody

Detection of Gelsolin/GSN in Human Renal Cell Carcinoma via seqIF™ staining on COMET™ Gelsolin/GSN Antibody was detected in immersion fixed paraffin-embedded sections of human Renal Cell Carcinoma using Mouse Anti-Human Gelsolin/GSN, pan Monoclonal Antibody (Catalog # MAB8170) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Detection of Human, Mouse, and Rat Gelsolin/GSN by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Gelsolin/GSN at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Gelsolin/GSN by Simple Western^TM. Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (U‑87 MG) (NBP2-49844) and SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Gelsolin/GSN at approximately 90 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Gelsolin/GSN in Human Kidney. Gelsolin/GSN was detected in formalin fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and distal convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Shows Human Gelsolin/GSN Specificity Using Knockout Cell Line. Western blot shows lysates of U2OS human osteosarcoma cell line and Gelsolin/GSN knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Gelsolin/GSN at approximately 80 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.