Human/Mouse/Rat GRIN2B/NMDAR2B Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of GRIN2B/NMDAR2B by Western Blot Western blot of 10 μg of rat brain hippocampus lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA Receptor. The labeling by the antibody was blocked by the C-terminal fusion protein of the NR2B subunit of the NMDA Receptor used as immunogen (not shown).
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Preparation and Storage
Background: GRIN2B/NMDAR2B
Rat NMDA receptor subunit 2B (NR2B) is a 1456 amino acid, 180 kDa, 3-transmembrane member of the glutamate-gated ion channel family. NMDA receptors are heteromultimers of at least two NR1 subunits and two NR2 or two (presumably) NR3 subunits. Upon ligand binding, NMDA receptors open and allow cation transit. The NR1 subunits bind glycine and are essential for the physical formation of the ion channel. NR2 subunits apparently bind glutamate and impart pharmacological properties to the receptor complex. Fully functional receptors require the presence of both glycine and glutamate. The NR1-NR2B complex has the highest affinity for glutamate. Both NR1 and NR2B subunits can be phosphorylated by protein kinase C (PKC). On NR1, PKC phosphorylates on serine at 890; on NR2B, PKC phosphorylates on tyrosine at 1472. Phosphorylation is considered to promote ion transit and impact NR2B interaction with downstream signaling molecules.
- Grosshans, D.R. and M.D. Browning (2001) J. Neurochem. 76:737.
- Cheung, H. et al. (2003) J. Neurochem. 86:1441.
- Gibb, A.J. (2004) Trends Neurosci. 27:7.
- Liu, Y. and J. Zhang (2000) Chin. Med. J. 113:948.
Product Datasheets
Citations for Human/Mouse/Rat GRIN2B/NMDAR2B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Altered hippocampal plasticity by prenatal kynurenine administration, kynurenine-3-monoxygenase (KMO) deletion or galantamine
Authors: C.M. Forrest, K. McNair, M. Pisar, O.S. Khalil, L.G. Darlington, T.W. Stone
Neuroscience
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Changes in synaptic transmission and protein expression in the brains of adult offspring after prenatal inhibition of the kynurenine pathway
Authors: C.M. Forrest, O.S. Khalil, M. Pisar, K. McNair, E. Kornisiuk, M. Snitcofsky et al.
Neuroscience
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Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring.
Authors: Forrest, Caroline, Khalil, Omari S, Pisar, Mazura, Smith, Robert A, Darlington, Lynda Ga, Stone, Trevor W
Mol Brain, 2012-06-09;5(0):22.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Reduced retinal function in amyloid precursor protein-over-expressing transgenic mice via attenuating glutamate-N-methyl-d-aspartate receptor signaling.
Authors: Shimazawa M, Inokuchi Y, Okuno T, Nakajima Y, Sakaguchi G, Kato A, Oku H, Sugiyama T, Kudo T, Ikeda T, Takeda M, Hara H
J. Neurochem., 2008-08-07;107(1):279-90.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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