Human/Mouse/Rat GRK1 Antibody

Catalog #: AF4498 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4498
AF4498-SP

Detection of Human/Mouse/Rat GRK1 by Western Blot.

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Human/Mouse/Rat GRK1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat GRK1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GRK1
Leu343-Ser563
Accession # Q15835

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat GRK1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitt's lymphoma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat GRK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4498) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRK1 at approximately 63 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRK1

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK1 and GRK7 are members of the rhodopsin kinase or visual GRK subfamily.

Long Name

G protein-coupled Receptor Kinase 1

Entrez Gene IDs

6011 (Human); 24013 (Mouse); 81760 (Rat)

Alternate Names

G protein-coupled receptor kinase 1rhodopsin kinase; GPRK1; GRK1; RHOK; RHOKEC 2.7.11; RKEC 2.7.11.14