Human/Mouse/Rat GRK7 Antibody

Catalog #: MAB4700 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4700
MAB4700-SP

Detection of Human, Mouse, and Rat GRK7 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat GRK7 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat GRK7 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 496831

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GRK7
Lys342-Leu553
Accession # Q8WTQ7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat GRK7 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, BJAB human Burkitt's lymphoma cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat GRK7 Monoclonal Antibody (Catalog # MAB4700) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRK7 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRK7

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183-188 amino acids and a carboxyl-terminus of variable length. GRK1 and GRK7 are members of the rhodopsin kinase or visual GRK subfamily. GRK7 is expressed in the cone outer segments of the retina and is able to phosphorylate rhodopsin in a light-dependent manner. In addition to its retina expression, GRK7 has been detected in other tissues, including brain, olfactory bulb, liver, lung and pancreas.

Long Name

G protein-coupled Receptor Kinase 7

Entrez Gene IDs

131890 (Human)

Alternate Names

EC 2.7.11; EC 2.7.11.16; G protein-coupled receptor kinase 7; GPRK7; GPRK7G protein-coupled receptor kinase GRK7; GRK7