Human/Mouse/Rat HCLS1 Antibody

Catalog #: AF5789 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5789
AF5789-SP

Detection of Human/Mouse/Rat HCLS1 by Western Blot.

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Human/Mouse/Rat HCLS1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat HCLS1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HCLS1
Met1-Gly84
Accession # P14317

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat HCLS1 by Western Blot. Western blot shows lysates of L1.2 mouse pro-B cell line, Nb2-11 rat lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat HCLS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5789) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HCLS1 at approximately 75 to 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HCLS1

HCLS1 (Hematopoietic lineage cell-specific protein; also HS1 and LckBP1) is a cytoplasmic substrate for non‑receptor type protein‑tyrosine kinases. Although its predicted MW is 54 kDa, it migrates anomalously at 75‑80 kDa in SDS-PAGE. HCLS1 is restricted to myeloid/lymphoid lineage cell types, including platelets, B cells, T cells and mast cells. HCLS1 binds to F-actin and promotes actin polymerization. It also serves as an intermediate for src-mediated signal transduction. Human HCLS1 is 486 amino acids (aa) in length. It contains one HAX1 binding site (aa 27‑66), three-plus cortactin type repeats (aa 79‑212), a coiled‑coil region (aa 215‑280) and an SH3 domain (aa 437‑484). HCLS1 is initially phosphorylated by Syk at Tyr378 and Tyr397, followed by a Src family kinase phosphorylation at Tyr222. This activates HCLS1, allowing it to interact with Ras-associated Grb2.

Long Name

Hematopoietic Cell-specific Lyn Substrate 1

Entrez Gene IDs

3059 (Human); 15163 (Mouse); 288077 (Rat)

Alternate Names

Cortactin-like; CTTNL; HCLS1; hematopoietic cell-specific Lyn substrate 1hematopoietic lineage cell-specific protein; HS1lckBP1; LckBP1; p75