Human/Mouse/Rat HSPA8/HSC71 Antibody Summary
Asp534-Gly615
Accession # P11142
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat HSPA8/HSC71 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat HSPA8/HSC71 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HSPA8/HSC71 at approximately 71 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
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HSPA8/HSC71 in Mouse Intestine. HSPA8/HSC71 was detected in immersion fixed frozen sections of mouse intestine using Goat Anti-Human/Mouse/Rat HSPA8/HSC71 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4148) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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HSPA8/HSC71 in Rat Intestine. HSPA8/HSC71 was detected in immersion fixed frozen sections of rat intestine using Goat Anti-Human/Mouse/Rat HSPA8/HSC71 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4148) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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HSPA8/HSC71 in Human Kidney. HSPA8/HSC71 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat HSPA8/HSC71 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4148) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSPA8/HSC71
The 70 kDa heat shock proteins (HSP70s) are a highly conserved family of stress response proteins. The HSP70 family of proteins contains both heat/stress inducible and constitutively expressed members known as heat shock cognate proteins. Heat Shock 70 kDa Protein 8 (HSPA8), also known as Heat Shock Cognate Protein 71 (HSC71), HSC70, HSP73, HSPA10 and LPS-associated protein 1 (LAP1), is a 646 amino acid (aa) heat shock cognate protein. Many HSPs function as molecular chaperones, facilitating the folding of other cellular proteins. HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding. HSPA8 is a ubiquitously expressed protein and rapidly translocates from the cytoplasm to the nucleus in response to heat shock. Two alternatively spliced variants have been identified. This antibody detects isoform 1 but not isoform 2 (HSC54). Human HSPA8 shares greater than 99% identity with mouse and rat HSPA8.
Product Datasheets
Citations for Human/Mouse/Rat HSPA8/HSC71 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Oncolytic Group B Adenovirus Enadenotucirev Mediates Non-apoptotic Cell Death with Membrane Disruption and Release of Inflammatory Mediators
Authors: A Dyer, Y Di, H Calderon, S Illingwort, G Kueberuwa, A Tedcastle, P Jakeman, SL Chia, A Brown, MA Silva, D Barlow, J Beadle, T Hermiston, DJ Ferguson, B Champion, KD Fisher, LW Seymour
Mol Ther Oncolytics, 2016-12-10;4(0):18-30.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery.
Authors: Katayama H, Kogure T, Mizushima N, Yoshimori T, Miyawaki A
Chem. Biol., 2011-08-26;18(8):1042-52.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Heat Shock Protein-70 Inhibition by the Small-Molecule 2-phenylethynesulfonamide Impairs Protein Clearance Pathways in Tumor Cells
Authors: J. I-Ju Leu, Julia Pimkina, Pooja Pandey, Maureen E. Murphy, Donna L. George
Molecular Cancer Research
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