Human/Mouse/Rat iNOS Antibody Summary
Pro781-His798
Accession # P35228
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human iNOS by Western Blot. Western blot shows lysates of DLD clone 2C2 human colon adenocarcinoma cell line untreated (-) or treated (+) with Recombinant Human IL-1 beta, Recombinant Human TNF-alpha, and Recombinant Human IFN-gamma (Catalog # HAF007). A specific band was detected for iNOS at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
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Detection of Mouse and Rat iNOS by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for iNOS at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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iNOS in Human Brain. iNOS was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Mouse iNOS by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS, loaded at 0.2 mg/mL. A specific band was detected for iNOS at approximately 136 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: iNOS
Inducible nitric oxide synthase (iNOS) is a widely expressed, approximately 130 kDa enzyme that catalyzes the conversion of L-arginine to nitric oxide (NO) plus citrulline. NO is a bioactive mediator that plays an important role in hemodynamics by inducing vascular smooth muscle relaxation. iNOS is constitutively expressed in the kidney. In other tissues, it is inducibly expressed during inflammation, oxidative stress, and hyperglycemia. iNOS activity is elevated in a variety of disease states including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Increased production of NO mediates acute kidney injury, TNF-alpha induced muscle wasting, and the increased radiosensitivity of hypoxic tumor cells. Within amino acids 781-798, human iNOS shares 89% and 72% aa sequence identity with mouse and rat iNOS, respectively.
Product Datasheets
Citations for Human/Mouse/Rat iNOS Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Pharmacological blockade of fatty acid amide hydrolase (FAAH) by URB597 improves memory and changes the phenotype of hippocampal microglia despite ethanol exposure
Authors: Rivera P, Fernandez-Arjona MDM, Silva-Pena D et al.
Biochem. Pharmacol.
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Exploring the Traditional Uses of Thymbra capitata Infusion in Algarve (Portugal): Anti-Inflammatory, Wound Healing, and Anti-Aging
Authors: Jorge Miguel Alves-Silva, Sónia Pedreiro, Maria Teresa Cruz, Lígia Salgueiro, Artur Figueirinha
Pharmaceuticals (Basel)
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Characterization of macrophage polarization in periodontal disease
Authors: Carlos Garaicoa‐Pazmino, Tobias Fretwurst, Cristiane H. Squarize, Tord Berglundh, William V. Giannobile, Lena Larsson et al.
Journal of Clinical Periodontology
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Synergistic Effects of Vitis vinifera L. and Centella asiatica against CCl4-Induced Liver Injury in Mice
Authors: Suvesh Munakarmi, Yamuna Gurau, Juna Shrestha, Prabodh Risal, Ho Sung Park, Geum-Hwa Lee et al.
International Journal of Molecular Sciences
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Grape seed proanthocyanidin inhibits inflammatory responses in hepatic stellate cells by modulating the MAPK, Akt and NF-kappa B signaling pathways
Authors: Jin-Woo Lee, Young Il Kim, Youngchul Kim, Minji Choi, Seoyeon Min, Yong Hoon Joo et al.
International Journal of Molecular Medicine
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Molecular Mechanisms of Immunomodulation Properties of Mesenchymal Stromal Cells: A New Insight into the Role of ICAM-1
Authors: Y Rubtsov, ? Goryunov, ? Romanov, Y Suzdaltsev, G Sharonov, V Tkachuk
Stem Cells Int, 2017-06-27;2017(0):6516854.
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Differential Expression of Kinin Receptors in Human Wet and Dry Age-Related Macular Degeneration Retinae
Authors: Rahmeh Othman, Simon Berbari, Elvire Vaucher, Réjean Couture
Pharmaceuticals (Basel)
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Inflammatory responses induced by Helicobacter pylori on the carcinogenesis of gastric epithelial GES‑1 cells
Authors: Jianjun Wang, Yongliang Yao, Qinghui Zhang, Shasha Li, Lijun Tang
International Journal of Oncology
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A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types
Authors: Fabian Gather, Irmgard Ihrig-Biedert, Paul Kohlhas, Tamara Krutenko, Michael Peitz, Oliver Brüstle et al.
Cell Communication and Signaling
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Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration
Authors: Jorge M. Alves-Silva, Sónia Pedreiro, Carlos Cavaleiro, Maria Teresa Cruz, Artur Figueirinha, Lígia Salgueiro
Nutrients
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Nrf2 orchestrates transition from acute to chronic otitis media through inflammatory macrophages
Authors: Wenyan Fan, Hongming Xu, Chenling Shen, Jia Fang, Xiaoyan Li
Frontiers in Immunology
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EZH2 Inhibitors Suppress Colorectal Cancer by Regulating Macrophage Polarization in the Tumor Microenvironment
Authors: Chen Li, Jiagui Song, Zhengyang Guo, Yueqing Gong, Tengrui Zhang, Jiaqi Huang et al.
Frontiers in Immunology
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Digital Spatial Profiling of Individual Glomeruli From Patients With Anti-Neutrophil Cytoplasmic Autoantibody-Associated Glomerulonephritis
Authors: Lin Ye, Yu Liu, Xuejing Zhu, Tongyue Duan, Chang Wang, Xiao Fu et al.
Frontiers in Immunology
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Atractylodes lancea (Thunb.) DC. [Asteraceae] rhizome-derived exosome-like nanoparticles suppress lipopolysaccharide-induced inflammation in murine microglial cells
Authors: Kawada, K;Ishida, T;Morisawa, S;Jobu, K;Higashi, Y;Aizawa, F;Yagi, K;Izawa-Ishizawa, Y;Niimura, T;Abe, S;Goda, M;Miyamura, M;Ishizawa, K;
Frontiers in pharmacology
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Arginine metabolism key enzymes affect the prognosis of myelodysplastic syndrome by interfering with macrophage polarization
Authors: Ou, Y;Yang, Y;Li, X;Zhang, X;Zhao, L;Yang, C;Wu, Y;
Cancer medicine
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Synthesis of Carvone Derivatives and In Silico and In Vitro Screening of Anti-Inflammatory Activity in Murine Macrophages
Authors: G Moço, C Sousa, A Capitão, SS MacKinnon, AJ Leitão, AF Mendes
International Journal of Molecular Sciences, 2023-01-23;24(3):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
In Vitro Characterization of a Tissue Renin-Angiotensin System in Human Nucleus Pulposus Cells
Authors: B Saravi, Z Li, V Basoli, S Grad, S Häckel, CE Albers, M Alini, H Schmal, P Obid, G Lang
Cells, 2022-10-28;11(21):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Harpagophytum procumbens Inhibits Iron Overload-Induced Oxidative Stress through Activation of Nrf2 Signaling in a Rat Model of Lumbar Spinal Stenosis
Authors: JY Hong, H Kim, J Lee, WJ Jeon, YJ Lee, IH Ha
Oxidative Medicine and Cellular Longevity, 2022-09-14;2022(0):3472443.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Insulin-like growth factor 2 promotes osteoclastogenesis increasing inflammatory cytokine levels under hypoxia
Authors: T Kondo, H Aoki, Y Otsuka, Y Kawaguchi, Y Waguri-Nag, M Aoyama
Journal of pharmacological sciences, 2022-04-16;149(3):93-99.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Ultrasound stimulation improves inflammatory resolution, neuroprotection, and functional recovery after spinal cord injury
Authors: YR Hong, EH Lee, KS Park, M Han, KT Kim, J Park
Scientific Reports, 2022-03-07;12(1):3636.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Anagliptin prevents lipopolysaccharide (LPS)- induced inflammation and activation of macrophages
Authors: F Yu, W Tian, J Dong
International immunopharmacology, 2022-01-16;104(0):108514.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Gongjin-Dan Enhances Neurite Outgrowth of Cortical Neuron by Ameliorating H2O2-Induced Oxidative Damage via Sirtuin1 Signaling Pathway
Authors: H Kim, W Jeon, J Hong, J Lee, C Yeo, Y Lee, S Baek, I Ha
Nutrients, 2021-11-27;13(12):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Senolytic Therapy for Cerebral Ischemia-Reperfusion Injury
Authors: S Lim, TJ Kim, YJ Kim, C Kim, SB Ko, BS Kim
International Journal of Molecular Sciences, 2021-11-04;22(21):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Sult2b1 deficiency exacerbates ischemic stroke by promoting pro-inflammatory macrophage polarization in mice
Authors: Y Wang, H Jin, Y Wang, Y Yao, C Yang, J Meng, X Tan, Y Nie, L Xue, B Xu, H Zhao, F Wang
Theranostics, 2021-11-01;11(20):10074-10090.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
BCG immunotherapy inhibits cancer progression by promoting the M1 macrophage differentiation of THP?1 cells via the Rb/E2F1 pathway in cervical carcinoma
Authors: L Liu, W Shi, X Xiao, X Wu, H Hu, S Yuan, K Liu, Z Liu
Oncology reports, 2021-09-28;46(5):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Migration and phenotype switching of macrophages at early-phase of bone-formation by secretomes from bone marrow derived mesenchymal stem cells using rat calvaria bone defect model
Authors: W Katagiri, R Takeuchi, N Saito, D Suda, T Kobayashi
Journal of dental sciences, 2021-08-31;17(1):421-429.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Neurotherapeutic Potential of Cervus elaphus Sibericus on Axon Regeneration and Growth Cone Reformation after H2O2-Induced Injury in Rat Primary Cortical Neurons
Authors: JY Hong, J Lee, H Kim, WJ Jeon, C Yeo, BR Choi, JE Yoon, JY Shin, JY Kim, IH Ha
Biology, 2021-08-26;10(9):.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface
Authors: A Chu, SY Kok, J Tsui, MC Lin, B Aguirre, M Wadehra
Journal of reproductive immunology, 2021-03-09;145(0):103309.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Study of fucoidans as natural biomolecules for therapeutical applications in osteoarthritis
Authors: C Vaamonde-G, N Flórez-Fer, MD Torres, MJ Lamas-Vázq, FJ Blanco, H Domínguez, R Meijide-Fa
Carbohydrate polymers, 2021-01-26;258(0):117692.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Anti-inflammatory potential of Portuguese thermal waters
Authors: A Silva, AS Oliveira, CV Vaz, S Correia, R Ferreira, L Breitenfel, J Martinez-d, R Palmeira-d, CMF Pereira, A Palmeira-d, MT Cruz
Scientific Reports, 2020-12-18;10(1):22313.
Species: Mouse
Sample Types: Cell Lysates
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Cancer-Derived Transforming Growth Factor-beta Modulates Tumor-Associated Macrophages in Ampullary Cancer
Authors: LC Cheng, YJ Chao, CY Wang, NN Phan, YL Chen, TW Wang, HP Hsu, YJ Lin, YS Shan, MD Lai
Onco Targets Ther, 2020-08-03;13(0):7503-7516.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Standardised comparison of limonene-derived monoterpenes identifies structural determinants of anti-inflammatory activity
Authors: C Sousa, AJ Leitão, BM Neves, F Judas, C Cavaleiro, AF Mendes
Sci Rep, 2020-04-29;10(1):7199.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Giardia lamblia Decreases NF-&kappaB p65RelA Protein Levels and Modulates LPS-Induced Pro-Inflammatory Response in Macrophages
Authors: CP Faria, BM Neves, Á Lourenço, MT Cruz, JD Martins, A Silva, S Pereira, MDC Sousa
Sci Rep, 2020-04-10;10(1):6234.
Species: N/A
Sample Types: Recombinant Protein
Applications: Western Blot -
Supplementation of endogenous Ahr ligands reverses insulin resistance and associated inflammation in an insulin-dependent diabetic mouse model
Authors: WC Liu, PH Chen, LW Chen
J. Nutr. Biochem., 2020-04-01;83(0):108384.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes
Authors: MAM Simões, DCGA Pinto, BMR Neves, AMS Silva
Molecules, 2020-02-13;25(4):.
Species: Mouse
Sample Types: Cell Culture Lysates
Applications: Western Blot -
The pro-inflammatory effect of NR4A3 in osteoarthritis
Authors: C Ma, L Wu, L Song, Y He, S Adel Abdo, S Yan, K Sheng, H Wu, J Ran, L Wu
J. Cell. Mol. Med., 2019-11-07;0(0):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery
Authors: W Ren, J Hou, C Yang, H Wang, S Wu, Y Wu, X Zhao, C Lu
J. Exp. Clin. Cancer Res., 2019-02-08;38(1):62.
Species: Xenograft
Sample Types: Cell Lysates
Applications: Western Blot -
Dimethyl sulfoxide but not indomethacin is efficient for healing in hydrofluoric acid eye burns
Authors: S Altan, Z O?urtan
Burns, 2016-10-27;43(1):232-244.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC -
Erythropoietin Receptor Antagonist Suppressed Ectopic Hemoglobin Synthesis in Xenografts of HeLa Cells to Promote Their Destruction.
Authors: Yasuda Y, Fujita M, Koike E, Obata K, Shiota M, Kotani Y, Musha T, Tsuji-Kawahara S, Satou T, Masuda S, Okano J, Yamasaki H, Okumoto K, Uesugi T, Nakao S, Hoshiai H, Mandai M
PLoS ONE, 2015-04-15;10(4):e0122458.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Artichoke, cynarin and cyanidin downregulate the expression of inducible nitric oxide synthase in human coronary smooth muscle cells.
Authors: Xia N, Pautz A, Wollscheid U, Reifenberg G, Forstermann U, Li H
Molecules, 2014-03-24;19(3):3654-68.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
High pre-transplant serum nitrate levels predict risk of acute steroid-refractory graft-versus-host disease in the absence of statin therapy.
Authors: Dietrich S, Okun J, Schmidt K, Falk C, Wagner A, Karamustafa S, Radujkovic A, Hegenbart U, Ho A, Dreger P, Luft T
Haematologica, 2013-10-18;99(3):541-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
BMC Neurosci, 2012-05-23;13(0):50.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
The autoantigen Ro52 is an E3 ligase resident in the cytoplasm but enters the nucleus upon cellular exposure to nitric oxide.
Authors: Espinosa A, Oke V, Elfving A, Nyberg F, Covacu R, Wahren-Herlenius M
Exp. Cell Res., 2008-09-25;314(20):3605-13.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P -
Proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease.
Authors: Skaleric U, Gaspirc B, McCartney-Francis N, Masera A, Wahl SM
Infect. Immun., 2006-10-02;74(12):7010-3.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Attenuation of apoptosis and enhancement of proteoglycan synthesis in rabbit cartilage defects by hyperbaric oxygen treatment are related to the suppression of nitric oxide production.
Authors: Yuan LJ, Ueng SW, Lin SS, Yeh WL, Yang CY, Lin PY
J. Orthop. Res., 2004-09-01;22(5):1126-34.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-P -
Implantation and tracing of green fluorescent protein-expressing adipose-derived stem cells in peri-implant capsular fibrosis
Authors: Bo-Yoon Park, Dirong Wu, Kyoo-Ri Kwon, Mi-Jin Kim, Tae-Gon Kim, Jun-Ho Lee et al.
Stem Cell Research & Therapy
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Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice
Authors: Heng Zhou, Pengfei Yang, Haining Li, Liying Zhang, Jin Li, Tianyi Zhang et al.
Cell Death Discovery
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Influenza A induces lactate formation to inhibit type I IFN in primary human airway epithelium
Authors: Jacob Thyrsted, Jacob Storgaard, Julia Blay-Cadanet, Alexander Heinz, Anne Laugaard Thielke, Stefania Crotta et al.
iScience
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Reducing Suppressors of Cytokine Signaling-3 (SOCS3) Expression Promotes M2 Macrophage Polarization and Functional Recovery After Intracerebral Hemorrhage
Authors: Xin-Chao Ji, Ya-Jun Shi, Yan Zhang, Ming-Ze Chang, Gang Zhao
Frontiers in Neurology
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Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis.
Authors: Parvu AE, Alb SF, Craciun A et al.
Acta Pharmacol Sin
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The Anti-Inflammatory Response of Lavandula luisieri and Lavandula pedunculata Essential Oils
Authors: Monica Zuzarte, Cátia Sousa, Carlos Cavaleiro, Maria Teresa Cruz, Lígia Salgueiro
Plants (Basel)
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Chemical Profile, Anti-Microbial and Anti-Inflammaging Activities of Santolina rosmarinifolia L. Essential Oil from Portugal
Authors: Jorge M. Alves-Silva, Maria José Gonçalves, Ana Silva, Carlos Cavaleiro, Maria Teresa Cruz, Lígia Salgueiro
Antibiotics (Basel)
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Leica Bond ER2 heat retrieval
primary Ab MAB9502 at 5ug/mL in BSA-TBS
Leica standard IHC protocol on Leica Rx stainer
Antibody also works very well for immunohistochemistry
Rats were perfused with 4% paraformaldehyde in PBS (pH=7.6). The brain was removed and immersed in PFA then in 30% sucrose. The brain was sliced with a freezing microtome. A mixture of 1% Triton-X and 10% donkey serum (in TRIS) was used for antigen retrieval and blocking.
Tmem119 antibody was used in 1:100 dilution and was visualized by a Cy3 conjugated anti-mouse secondary antibody (1:500, yellow). Cell bodies were stained with NeuroTrace 640/660 (1:250, red). Pictures show a part of the rat hippocampus.
The product was assumed to label activated microglial cells. It seems, it did not. However, it labelled some cerebral blood vessels, which is also meaningful, thus, it may be useful for this application.
