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Human/Mouse/Rat Keap1 Antibody

Catalog #: MAB3024
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3024
MAB3024-SP
Detection of Human/Mouse/Rat Keap1 by Western Blot.
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Product Details
Citations (6)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Keap1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Keap1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 333116
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Keap1
Ala90-Ile250
Accession # Q14145
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
See below
Knockout Validated
Keap1 is specifically detected in Jurkat human acute T cell leukemia parental cell line but is not detectable in Keap1 knockout Jurkat cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Keap1 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Keap1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Keap1 Monoclonal Antibody (Catalog # MAB3024) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Keap1 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Simple Western Detection of Human Keap1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Keap1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Keap1 at approximately 75 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Keap1 Monoclonal Antibody (Catalog # MAB3024). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Knockout Validated Western Blot Shows Human Keap1 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Keap1 Specificity by Using Knockout Cell Line. Western blot shows lysates of Jurkat human acute T cell leukemia parental cell line and Keap1 Jurkat cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Keap1 Monoclonal Antibody (Catalog # MAB3024) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Keap1 at approximately 66 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in knockout Jurkat cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Keap1

Kelch-like ECH-associated protein 1 (Keap1) is a 624 amino acid, 69 kDa protein which interacts with the transcription factor NF-E2-related factor 2 (Nrf2). Keap1 represses Nrf2 function by sequestering Nrf2 in the cytoplasm. Keap1 contains an N-terminal BTB domain and six C-terminal KELCH domains (aa 327‑611) that interact with Nrf2. Dissociation of the two proteins in response to redox-sensitive cell stress is followed by the translocation of Nrf2 to the nucleus and transcription of detoxifying/oxidative stress enzyme genes.

Long Name
Kelch-like ECH-associated Protein 1
Entrez Gene IDs
9817 (Human)
Alternate Names
INrf2; INrf2MGC9454; Keap1; kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132MGC4407; KLHL19Cytosolic inhibitor of Nrf2; MGC10630; MGC1114; MGC20887

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Citations for Human/Mouse/Rat Keap1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Antioxidant and Anti-Inflammatory Properties of Rubber Seed Oil in Lipopolysaccharide-Induced RAW 267.4 Macrophages
    Authors: Jing Liu, Lulu Zhao, Hongying Cai, Zitao Zhao, Yongbao Wu, Zhiguo Wen et al.
    Nutrients
  2. Risperidone ameliorates prefrontal cortex neural atrophy and oxidative/nitrosative stress in brain and peripheral blood of rats with neonatal ventral hippocampus lesion
    Authors: H Tendilla-B, S Meneses-Pr, R Antonio Vá, M Tapia-Rodr, A Judith Váz, H Coatl-Cuay, D Martín-Her, KS MacDowell, L Garcés-Ram, JC Leza, G Flores
    J. Neurosci., 2019-09-13;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Nrf2-Keap1 pathway promotes cell proliferation and diminishes ferroptosis
    Authors: Z Fan, AK Wirth, D Chen, CJ Wruck, M Rauh, M Buchfelder, N Savaskan
    Oncogenesis, 2017-08-14;6(8):e371.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Decreased DJ-1 Leads to Impaired Nrf2-Regulated Antioxidant Defense and Increased UV-A–Induced Apoptosis in Corneal Endothelial Cells
    Authors: Cailing Liu, Yuming Chen, Irene E. Kochevar, Ula V. Jurkunas
    Investigative Opthalmology & Visual Science
  5. Deficiency in the nuclear factor E2-related factor-2 transcription factor results in impaired adipogenesis and protects against diet-induced obesity.
    Authors: Pi J, Leung L, Xue P, Wang W, Hou Y, Liu D, Yehuda-Shnaidman E, Lee C, Lau J, Kurtz TW, Chan JY
    J. Biol. Chem., 2010-01-20;285(12):9292-300.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Modulation of Keap1/Nrf2/ARE Signaling Pathway by Curcuma- and Garlic-Derived Hybrids
    Authors: Serafini M, Catanzaro M, Fagiani F et al.
    Front. Pharmacol.

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