Home / LHX5 / Human/Mouse/Rat LHX5 Antibody

Human/Mouse/Rat LHX5 Antibody

Catalog #: AF6290
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6290
AF6290-SP
Detection of Rat LHX5 by Western Blot.
2 Images
Product Details
Citations (5)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat LHX5 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects recombinant human LHX5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LIM1 is observed, and less than 5% cross-reactivity with rhLHX2, and rhLHX9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LHX5
Leu265-Trp402
Accession # Q9H2C1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat LHX5 antibody by Western Blot. View Larger

Detection of Rat LHX5 by Western Blot. Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LHX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6290) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LHX5 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry LHX5 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

LHX5 in Mouse Brain. LHX5 was detected in perfusion fixed frozen sections of adult mouse brain (hippocampus) using Goat Anti-Human LHX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6290) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LHX5

LHX5 (LIM/homeobox protein 5) is a member of the LIM homeobox gene family. It is expressed in embryonic CNS, and appears to contribute to the formation and maintenance of multiple neuronal phenotypes. In the cerebellum, LHX5 promotes Purkinje cell development; in the spinal cord, LHX5 maintains a GABAergic phenotype in select dorsal horn interneurons. LHX5 is reported to act as a Wnt pathway antagonist through its stimulation of sFRP-1 and -5 secretion. Human LHX5 is 402 amino acids (aa) in length. It contains two LIM Zn-binding domains (aa 3-61 and 62-125), one phosphorylation site at Tyr119, and a DNA-binding homeodomain (aa 180‑239). Over aa 265-402, human LHX5 shares 97% aa identity with mouse LHX5, and 60% aa identity with its paralogue (common ancestoral) gene, LHX1.

Long Name
LIM (Lin11:Isl1:Mec3)/Homeobox Protein 5
Entrez Gene IDs
64211 (Human); 16873 (Mouse); 124451 (Rat)
Alternate Names
LHX5; LIM homeobox 5; LIM homeobox protein 5; LIM/homeobox protein Lhx5; MGC129689

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Citations for Human/Mouse/Rat LHX5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. NOVA regulate Dcc alternative splicing during neuronal migration and axon guidance in the spinal cord.
    Authors: Leggere JC, Saito Y, Darnell RB et al.
    Elife.
  2. Lactate-dependent transcriptional regulation controls mammalian eye morphogenesis
    Authors: Takata, N;Miska, JM;Morgan, MA;Patel, P;Billingham, LK;Joshi, N;Schipma, MJ;Dumar, ZJ;Joshi, NR;Misharin, AV;Embry, RB;Fiore, L;Gao, P;Diebold, LP;McElroy, GS;Shilatifard, A;Chandel, NS;Oliver, G;
    Nature communications
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia
    Authors: Ugomma C. Eze, Aparna Bhaduri, Maximilian Haeussler, Tomasz J. Nowakowski, Arnold R. Kriegstein
    Nature Neuroscience
  4. PRC2 Facilitates the Regulatory Topology Required for Poised Enhancer Function during Pluripotent Stem Cell Differentiation
    Authors: S Cruz-Molin, P Respuela, C Tebartz, P Kolovos, M Nikolic, R Fueyo, WF van Ijcken, F Grosveld, P Frommolt, H Bazzi, A Rada-Igles
    Cell Stem Cell, 2017-03-09;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Netrin1/DCC signaling promotes neuronal migration in the dorsal spinal cord.
    Authors: Junge HJ, Yung AR, Goodrich LV, Chen Z.
    Neural Dev.

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