Human/Mouse/Rat Myoglobin Antibody

Catalog #: MAB97203 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB97203-100
MAB97203-SP

Detection of Human, Mouse, and Rat Myoglobin by Western Blot.

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All Myoglobin Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Myoglobin Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Myoglobin in direct ELISAs. Detects human, mouse, and rat Myoglabin in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2269B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Purifed human cardiac Myoglobin antigen from human heart
Accession # P02144

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

10 µg/mL

See below

ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97204).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunohistochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Myoglobin by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, rat heart tissue, and human liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97203) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Myoglobin at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Myoglobin in Human Heart. Myoglobin was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97203) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western

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Detection of Human, Mouse, and Rat Myoglobin by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue, mouse heart tissue, rat heart tissue, and human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Myoglobin at approximately 21-24 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97203). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

ELISA

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Human Myoglobin ELISA Standard Curve. Recombinant Human Myoglobin protein was serially diluted 2-fold and captured by Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97204) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human/Mouse/Rat Myoglobin Monoclonal Antibody (Catalog # MAB97203) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myoglobin

Myoglobin is a 17KDa cytoplasmic oxygen-binding protein encoded by the MB gene and expressed in myocytes of the heart and skeletal muscle. Its name derives from its structural and functional similarity to hemoglobin, the oxygen binding protein found in red blood cells. Functions of myoglobin include oxygen storage and transport, as well as scavenging of NO and reactive oxygen species. Myoglobin also serves as a sensitive marker for muscle injury resulting from cardiac infarction. Myoglobin was the first protein to have its three-dimensional structure determined by X-ray crystallography.

Entrez Gene IDs

4151 (Human); 17189 (Mouse); 59108 (Rat)

Alternate Names

MB; MGC13548; Myoglobin; PVALB