Human/Mouse/Rat NEDD4 Antibody Summary
Asn211-Gly349 (predicted)
Accession # NP_006145
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat NEDD4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT-2 mouse T cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human NEDD4 Monoclonal Antibody (Catalog # MAB6218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEDD4 at approximately 118 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NEDD4
NEDD4 (Neural precursor cell-expressed developmentally down-regulated gene 4-1; also RPF1) is a 115-120 kDa member of the HECT E3 ubiquitin ligase family of proteins. The NEDD4 gene is widely expressed, and serves as a complement to a related gene product termed NEDD4-2/NEDD4L. Both molecules ubiquitinate multiple proteins. NEDD4-1 acts preferentially on PTEN, tumor suppressors and endocytic proteins, while NEDD4-2 acts preferentially on membrane transporters. Human NEDD4-1 is 900 amino acids (aa) in length. It contains one PKC region (aa 20-124), four WW domains (aa 196-505) and one E3 HECT domain (aa 543-897). Caspase cleavage after Asp197 generates a 95 kDa C-terminal, and smaller but variable sized N-terminal fragment. There are multiple splice forms. Two show a 516 aa substitution for aa 1-169 and 1-97, respectively, while a third shows the latter substitution coupled to a deletion of aa 170-185. Over aa 211-349, human NEDD4-1 shares 83% aa identity to mouse NEDD4-1 and less than 25% aa identity with human NEDD4-2.
Product Datasheets
Citations for Human/Mouse/Rat NEDD4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Nedd4 E3 ligase and beta-arrestins regulate ubiquitination, trafficking, and stability of the mGlu7 receptor
Authors: Sanghyeon Lee, Sunha Park, Hyojin Lee, Seulki Han, Jae-man Song, Dohyun Han et al.
eLife
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Inhibition of histone methyltransferase Smyd3 rescues NMDAR and cognitive deficits in a tauopathy mouse model
Authors: JB Williams, Q Cao, W Wang, YH Lee, L Qin, P Zhong, Y Ren, K Ma, Z Yan
Nature Communications, 2023-01-06;14(1):91.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions
Authors: Nobre LV, Nightingale K, Ravenhill BJ et al.
Elife
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