Human/Mouse/Rat NEDP1/SENP8 Antibody

Catalog #: AF7760 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7760
AF7760-SP

Detection of Human, Mouse, and Rat NEDP1/SENP8 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat NEDP1/SENP8 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, rat NEDP1/SENP8 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NEDP1/SENP8
Met1-Lys212 (Thr207Ala)
Accession # Q96LD8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat NEDP1/SENP8 by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line, beta TC-6 mouse beta cell insulinoma cell line, and NRK-49F rat fibroblasts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat NEDP1/SENP8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7760) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NEDP1/SENP8 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NEDP1/SENP8

NEDP1 (NEDD8-specific protease 1; also DEN1/Deneddylase-1 Protease, cysteine 2 and SENP8/Sentrin-specific protease 8) is a 24-26 kDa cytoplasmic cysteine protease that belongs to the Ulp/peptidase C48 family of enzymes. It is widely expressed, and is involved in NEDD8-associated ubiquitination. NEDD8 is a 9 kDa polypeptide that covalently binds to, and activates cullin-1, a molecule associated with a ubiquitin ligase complex. NEDD8-activated cullin promotes the transfer of ubiquitin to a limited number of target substrates involved in the cell cycle. This has the effect of controlling the levels of cell cycle regulators within the cell. NEDP1 influences these activities in two ways; first, it converts preNEDD8 into its mature form, and second, it deconjugates NEDD8 from its bound substrates. Human NEDP1 is 212 amino acids (aa) in length. It possesses an acetylated initiating methionine plus a protease domain that spans aa 11-174. Over an equivalent sequence, full-length human NEDP1 shares 92% aa sequence identity with mouse NEDP1. The mouse NEDP1 molecule contains a nine aa extension at the C-terminus not shared by the human molecule.

Entrez Gene IDs

123228 (Human); 71599 (Mouse); 315723 (Rat)

Alternate Names

DEN1; DEN1deneddylase-1; deneddylase 1; Deneddylase-1; EC 3.4.22.-; HsT17512; NEDD8-specific protease 1; NEDP1; NEDP1NEDD8 specific-protease cysteine 2; Protease, cysteine 2; protease, cysteine, 2 (NEDD8 specific); PRSC2; SENP8; Sentrin/SUMO-specific protease SENP8; sentrin-specific protease 8; SUMO sentrin specific protease family member 8; SUMO/sentrin specific peptidase family member 8; SUMO/sentrin specific protease family member 8