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Human/Mouse/Rat Neurofascin Antibody

Catalog #: AF3235
32 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3235
AF3235-SP
Detection of Human, Mouse, and Rat Neurofascin by Western Blot.
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Citations (32)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Neurofascin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, rat Neurofascin in Western blots and rat Neurofascin in direct ELISAs.
Source
Polyclonal Chicken IgY
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neurofascin
Ile25-Ala1031
Accession # NP_446361
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Neurofascin antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Neurofascin by Western Blot. Western blot shows lysates of rat brain tissue, mouse brain tissue, and human brain (cerebellum). PVDF Membrane was probed with 0.1 µg/mL of Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. Specific bands were detected for Neurofascin at approximately 140 and186 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Neurofascin antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

Neurofascin in Rat Brain. Neurofascin was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglion) using Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (yellow; Catalog # NL016) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and Schwann cells (perinodal regions). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurofascin

Neurofascin 155 (NF155) is a type I transmembrane glycoprotein that belongs to the L1CAM family of cell adhesion proteins (1, 2). The rat NF155 cDNA encodes a 1240 amino acid (aa) precursor that contains a 24 aa signal sequence, a 1086 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 109 aa cytoplasmic domain. The ECD consists of six Ig-like domains and four fibronectin type III repeats, the second of which has an RGD motif. A splice variant of Neurofascin, NF186, lacks the RGD-containing fibronectin type III domain but instead has a mucin-like domain and an additional non-RGD fibronectin type III domain (3). Within shared regions of the ECD, rat NF155 shares 45% and 39% aa sequence identity with rat Nr-CAM and L1CAM, respectively, and 98% aa sequence identity with human and mouse NF155. NF155 is transiently expressed by oligodendrocytes at the onset of axon myelination, whereas NF186 is neuronally expressed in nodes of Ranvier (4‑6). Clustering of NF155 in paranodal oligodengroglia lipid raft domains is stabilized by dimerization of its cytoplasmic domains and association with intracellular ankyrin (6‑9). NF155 interacts with axonal contactin and plays a role in node of Ranvier formation and the establishment of saltatory conduction (5, 9‑12). The ECD of NF155 is cleaved from oligodengroglia membranes by metalloproteases, a process which is required for NF155 transport from the glial cell body to the axoglial junction (13). In addition to distinct expression patterns, Neurofascin isoforms have different functional properties. NF155 promotes neuronal adhesion and neurite outgrowth, whereas NF186 inhibits neuronal adhesion (4, 7, 13).

References
  1. Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
  2. Coman, I. et al. (2005) J. Neurol. Sci. 233:67.
  3. Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
  4. Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
  5. Collinson, J.M. et al. (1998) Glia 23:11.
  6. Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
  7. Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
  8. Zhang, X. et al. (1998) J. Biol. Chem. 273:30785.
  9. Sherman, D.L. et al. (2005) Neuron 48:737.
  10. Gollan, L. et al. (2003) J. Cell Biol. 163:1213.
  11. Charles, P. et al. (2002) Curr. Biol. 12:217.
  12. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  13. Maier, O. et al. (2006) Exp. Cell Res. 312:500.
Entrez Gene IDs
23114 (Human); 269116 (Mouse); 116690 (Rat)
Alternate Names
DKFZp686P2250; KIAA0756FLJ46866; neurofascin homolog (chicken); neurofascin homolog; Neurofascin; NF; NFASC; NRCAML

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Citations for Human/Mouse/Rat Neurofascin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Reduced length of nodes of Ranvier and altered proteoglycan immunoreactivity in prefrontal white matter in major depressive disorder and chronically stressed rats
    Authors: José Javier Miguel-Hidalgo, Erik Hearn, Mohadetheh Moulana, Khunsa Saleem, Austin Clark, Maggie Holmes et al.
    Sci Rep
  2. Loss of TMEM106B and PGRN leads to severe lysosomal abnormalities and neurodegeneration in mice
    Authors: Feng T, Mai S, Roscoe JM et al.
    EMBO Rep.
  3. Antibodies against nodo-paranodal proteins are not present in genetic neuropathies
    Authors: Lorena Martín-Aguilar, Elba Pascual-Goñi, Cinta Lleixà, Marina Frasquet, Herminia Argente, Angel Cano-Abascal et al.
    Neurology
  4. The Polarity Protein Pals1 Regulates Radial Sorting of Axons
    Authors: Daniel R Zollinger, Kae-Jiun Chang, Kelli Baalman, Seonhee Kim, Matthew N Rasband
    Journal of Neuroscience
  5. Type 2 Diabetes Leads to Axon Initial Segment Shortening in db/db Mice
    Authors: Leonid M. Yermakov, Domenica E. Drouet, Ryan B. Griggs, Khalid M. Elased, Keiichiro Susuki
    Frontiers in Cellular Neuroscience
  6. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K(+) channels
    Authors: Stevens SR, Longley CM, Ogawa Y et al.
    eLife
  7. beta spectrin-dependent and domain specific mechanisms for Na+ channel clustering
    Authors: Cheng-Hsin Liu, Ryan Seo, Tammy Szu-Yu Ho, Michael Stankewich, Peter J Mohler, Thomas J Hund et al.
    eLife
  8. Diabetes Mellitus Is a Possible Risk Factor for Nodo-paranodopathy With Antiparanodal Autoantibodies
    Authors: Luise Appeltshauser, Julia Messinger, Katharina Starz, David Heinrich, Anna-Michelle Brunder, Helena Stengel et al.
    Neurology - Neuroimmunology Neuroinflammation
  9. Glial M6B stabilizes the axonal membrane at peripheral nodes of Ranvier
    Authors: Marie L Bang, Anya Vainshtein, Hyun-Jeong Yang, Yael Eshed-Eisenbach, Jerome Devaux, Hauke B Werner et al.
    Glia
  10. Endogenously expressed Ranbp2 is not at the axon initial segment
    Authors: Yuki Ogawa, Matthew N. Rasband
    Journal of Cell Science
  11. Antibody-directed extracellular proximity biotinylation reveals that Contactin-1 regulates axo-axonic innervation of axon initial segments
    Authors: Ogawa, Y;Lim, BC;George, S;Oses-Prieto, JA;Rasband, JM;Eshed-Eisenbach, Y;Hamdan, H;Nair, S;Boato, F;Peles, E;Burlingame, AL;Van Aelst, L;Rasband, MN;
    Nature communications
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  12. Compromised Myelin and Axonal Molecular Organization Following Adult-Onset Sulfatide Depletion
    Authors: Dustin, E;Suarez-Pozos, E;Stotesberry, C;Qiu, S;Palavicini, JP;Han, X;Dupree, JL;
    Biomedicines
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Antibody-directed extracellular proximity biotinylation reveals Contactin-1 regulates axo-axonic innervation of axon initial segments
    Authors: Y Ogawa, BC Lim, S George, JA Oses-Priet, JM Rasband, Y Eshed-Eise, S Nair, F Boato, E Peles, AL Burlingame, LV Aelst, MN Rasband
    bioRxiv : the preprint server for biology, 2023-03-06;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Biotinylation, ICC
  14. Distinct Changes in Calpain and Calpastatin during PNS Myelination and Demyelination in Rodent Models
    Authors: JA Miller, DE Drouet, LM Yermakov, MS Elbasiouny, FZ Bensabeur, M Bottomley, K Susuki
    International Journal of Molecular Sciences, 2022-12-06;23(23):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  15. A new mouse model of Charcot-Marie-Tooth 2J neuropathy replicates human axonopathy and suggest alteration in axo-glia communication
    Authors: G Shacklefor, LN Marziali, Y Sasaki, A Claessens, C Ferri, NI Weinstock, AM Rossor, NJ Silvestri, ER Wilson, E Hurley, GJ Kidd, S Manohar, D Ding, RJ Salvi, ML Feltri, M D'Antonio, L Wrabetz
    PloS Genetics, 2022-11-09;18(11):e1010477.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K(+) channels
    Authors: Stevens SR, Longley CM, Ogawa Y et al.
    eLife
  17. Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells
    Authors: G Della-Flor, ER Wilson, LN Marziali, E Hurley, N Silvestri, B He, BW O'Malley, B Beirowski, Y Poitelon, L Wrabetz, ML Feltri
    Nature Communications, 2021-06-02;12(1):3285.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells
    Authors: KJ Chang, I Agrawal, A Vainshtein, WY Ho, W Xin, G Tucker-Kel, K Susuki, E Peles, SC Ling, JR Chan
    Elife, 2021-03-10;10(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Immunoadsorption and Plasma Exchange in Seropositive and Seronegative Immune-Mediated Neuropathies
    Authors: AJ Davies, J Fehmi, M Senel, H Tumani, J Dorst, S Rinaldi
    J Clin Med, 2020-06-27;9(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Accumulation of Neurofascin at nodes of Ranvier is regulated by a Paranodal Switch
    Authors: Y Zhang, S Yuen, E Peles, JL Salzer
    J. Neurosci., 2020-06-17;0(0):.
    Species: Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Glial ?II spectrin contributes to paranode formation and maintenance
    Authors: K Susuki, DR Zollinger, KJ Chang, C Zhang, CY Huang, CR Tsai, MR Galiano, Y Liu, SD Benusa, LM Yermakov, RB Griggs, JL Dupree, MN Rasband
    J. Neurosci., 2018-05-31;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Methylglyoxal Disrupts Paranodal Axoglial Junctions via Calpain Activation
    Authors: RB Griggs, LM Yermakov, DE Drouet, DVM Nguyen, K Susuki
    ASN Neuro, 2018-01-01;10(0):1759091418766.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Antibodies against peripheral nerve antigens in chronic inflammatory demyelinating polyradiculoneuropathy
    Authors: L Querol, AM Siles, R Alba-Rovir, A Jáuregui, J Devaux, C Faivre-Sar, J Araque, R Rojas-Garc, J Diaz-Maner, E Cortés-Vic, G Nogales-Ga, M Navas-Madr, E Gallardo, I Illa
    Sci Rep, 2017-10-31;7(1):14411.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. The paranodal cytoskeleton clusters Na(+) channels at nodes of Ranvier
    Authors: V Amor, C Zhang, A Vainshtein, A Zhang, DR Zollinger, Y Eshed-Eise, PJ Brophy, MN Rasband, E Peles
    Elife, 2017-01-30;6(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Nodal beta spectrins are required to maintain Na+ channel clustering and axon integrity
    Authors: Liu CH, Stevens SR, Teliska LH et al.
    Elife
  26. Neurofascin Is a Novel Component of Rod Photoreceptor Synapses in the Outer Retina
    Authors: Sahar Pourhoseini, Debalina Goswami-Sewell, Elizabeth Zuniga-Sanchez
    Frontiers in Neural Circuits
  27. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier
    Authors: Tammy Szu-Yu Ho, Daniel R. Zollinger, Kae-Jiun Chang, Mingxuan Xu, Edward C. Cooper, Michael C. Stankewich et al.
    Nature Neuroscience
  28. Glial ankyrins facilitate paranodal axoglial junction assembly
    Authors: Kae-Jiun Chang, Daniel R. Zollinger, Keiichiro Susuki, Diane L. Sherman, Michael A. Makara, Peter J. Brophy et al.
    Nature Neuroscience
  29. An alpha II Spectrin-Based Cytoskeleton Protects Large-Diameter Myelinated Axons from Degeneration
    Authors: Claire Yu-Mei Huang, Chuansheng Zhang, Daniel R. Zollinger, Christophe Leterrier, Matthew N. Rasband
    The Journal of Neuroscience
  30. NuMA1 promotes axon initial segment assembly through inhibition of endocytosis
    Authors: Tomohiro Torii, Yuki Ogawa, Cheng-Hsin Liu, Tammy Szu-Yu Ho, Hamdan Hamdan, Chih-Chuan Wang et al.
    J. Cell Biol
  31. Impairment of cognitive flexibility in type 2 diabetic db/db mice
    Authors: Leonid M. Yermakov, Ryan B. Griggs, Domenica E. Drouet, Chiho Sugimoto, Michael T. Williams, Charles V. Vorhees et al.
    Behavioural Brain Research
  32. m6A mRNA Methylation Is Essential for Oligodendrocyte Maturation and CNS Myelination
    Authors: Huan Xu, Yulia Dzhashiashvili, Ankeeta Shah, Rejani B. Kunjamma, Yi-lan Weng, Benayahu Elbaz et al.
    Neuron

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