Human/Mouse/Rat Nucleostemin Antibody Summary
Lys2-Ile538
Accession # Q811S9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human Nucleostemin by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nucleostemin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Nucleostemin in U2OS Human Cell Line. Nucleostemin was detected in immersion fixed U2OS human osteosarcoma cell line using 10 µg/mL Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Nucleostemin by Simple WesternTM. Simple Western lane view shows lysates of PC-3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Nucleostemin at approximately 83 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human Nucleostemin by Immunocytochemistry/Immunofluorescence Embryonic stem cell markers. Immunohistochemistry: A. NANOG; B. OCT4. The majority of nuclei in the NANOG IHC are brown indicating the presence of NANOG. Some, but not all, nuclei are positive for OCT4. Negative (secondary antibody only) and positive (seminoma) controls are presented in the thumbnails below the photomicrographs. C. Immunofluorescence against nucleostemin. Left: anti-nucleostemin antibody, center: DAPI, right: merge. Bar = 10 microns. Image collected and cropped by CiteAb from the following publication (https://bmcmolcellbiol.biomedcentral.com/articles/10.1186/1471-2121-15-20), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nucleostemin
Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells (1-3).
- Tsai, R.Y. and R.D. McKay (2002) Genes Dev. 16:2991.
- Baddoo, M. et al. (2003) J. Cell Biochem. 89:1235.
- Normile, D. (2002) Science 298:1869.
Product Datasheets
Citations for Human/Mouse/Rat Nucleostemin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Immunohistochemical study of doublecortin and nucleostemin in canine brain
Authors: E. De Nevi, P. Marco-Salazar, D. Fondevila,1, E. Blasco, L. Pérez, M. Pumarola
European Journal of Histochemistry
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Viral targeting of glioblastoma stem cells with patient-specific genetic and post-translational p53 deregulations
Authors: J Gil-Ranedo, C Gallego-Ga, JM Almendral
Cell Reports, 2021-09-07;36(10):109673.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Overexpression of nucleostemin contributes to an advanced malignant phenotype and a poor prognosis in oral squamous cell carcinoma.
Authors: Yoshida, R, Nakayama, H, Nagata, M, Hirosue, A, Tanaka, T, Kawahara, K, Nakagawa, Y, Matsuoka, Y, Sakata, J, Arita, H, Hiraki, A, Shinohara, M, Ito, T
Br J Cancer, 2014-10-14;111(12):2308-15.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
Phenotypic plasticity in normal breast derived epithelial cells.
Authors: Sauder C, Koziel J, Choi M, Fox M, Grimes B, Badve S, Blosser R, Radovich M, Lam C, Vaughan M, Herbert B, Clare S
BMC Cell Biol, 2014-06-10;15(0):20.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Nucleostemin is indispensable for the maintenance and genetic stability of hematopoietic stem cells.
Authors: Yamashita M, Nitta E, Nagamatsu G, Ikushima Y, Hosokawa K, Arai F, Suda T
Biochem Biophys Res Commun, 2013-10-16;441(1):196-201.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC-Fr -
Reactive oxygen species regulate nucleostemin oligomerization and protein degradation.
Authors: Huang M, Whang P, Chodaparambil JV
J. Biol. Chem., 2011-01-17;286(13):11035-46.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Depletion of guanine nucleotides leads to the Mdm2-dependent proteasomal degradation of nucleostemin.
Authors: Huang M, Itahana K, Zhang Y, Mitchell BS
Cancer Res., 2009-03-24;69(7):3004-12.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Identification of Novel Markers That Demarcate the Nucleolus during Severe Stress and Chemotherapeutic Treatment
Authors: Haitong Su, Mohamed Kodiha, Sunghoon Lee, Ursula Stochaj
PLoS ONE
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Gold Nanoparticles Impinge on Nucleoli and the Stress Response in MCF7 Breast Cancer Cells
Authors: Mohamed Kodiha, Hicham Mahboubi, Dusica Maysinger, Ursula Stochaj
Nanobiomedicine (Rij)
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