Human/Mouse/Rat Pannexin-1 Antibody

Catalog #: MAB7097
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7097
MAB7097-SP

Detection of Human, Mouse, and Rat Pannexin‑1 by Western Blot.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Pannexin-1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Pannexin-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Pannexin-2 or
-3 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 720505

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Pannexin-1
Pro298-Asp376
Accession # Q96RD7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Pannexin‑1 by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Pannexin-1 Monoclonal Antibody (Catalog # MAB7097) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pannexin-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pannexin-1

Pannexin-1 (PANX1) is an approximately 45 kDa member of the Pannexin family of four-transmembrane channel proteins with a conserved pattern of cysteines. It is expressed on epithelial cells, neuronal and glial cells, cardiac myocytes, T cells, and erythrocytes. Pannexin-1 forms large pore hexameric channels or heteromeric channels with Pannexin-2 and enables the efflux of ATP and UTP. Its conductance is increased in response to hypoxia, isotonic stress, NMDA R activation, and Caspase-3 and -7 activation during apopotosis. Extracellular release of ATP through Pannexin-1 activates P2X7 receptors which in turn induces the closure of Pannexin-1 channels. Pannexin-1 cooperates with P2X7 for inflammasome activation, Caspase-1 activation, and the release of mature IL-1 beta and also regulates T cell activation at the immunological synapse. Within aa 298-376 (in the C-terminal cytoplasmic domain), human Pannexin-1 shares 96% and 98% aa sequence identity with mouse and rat Pannexin-1, respectively. Alternate splicing generates an additional isoform that lacks the C-terminal four amino acids in the cytoplasmic tail.

Entrez Gene IDs

24145 (Human); 55991 (Mouse); 315435 (Rat)

Alternate Names

Innexin; MGC21309; MRS1; MRS1innexin; pannexin 1; Pannexin1; Pannexin-1; PANX1; PX1; UNQ2529

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human/Mouse/Rat Pannexin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Adaptive thermogenesis in brown adipose tissue involves activation of pannexin-1 channels
Authors: S Senthivina, V Serbulea, CM Upchurch, R Polanowska, SK Mendu, S Sahu, P Jayaguru, KW Aylor, MD Chordia, L Steinberg, N Oberholzer, S Uchiyama, N Inada, UM Lorenz, TE Harris, SR Keller, AK Meher, A Kadl, BN Desai, BK Kundu, N Leitinger
Mol Metab, 2020-11-25;0(0):101130.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Acute Pannexin 1 Blockade Mitigates Early Synaptic Plasticity Defects in a Mouse Model of Alzheimer's Disease
Authors: C Flores-Muñ, B Gómez, E Mery, P Mujica, I Gajardo, C Córdova, D Lopez-Espí, C Durán-Anio, C Hetz, P Muñoz, AM Gonzalez-J, ÁO Ardiles
Front Cell Neurosci, 2020-03-19;14(0):46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Novel evidence that extracellular nucleotides and purinergic signaling induce innate immunity-mediated mobilization of hematopoietic stem/progenitor cells
Authors: M Adamiak, K Bujko, M Cymer, M Plonka, T Glaser, M Kucia, J Ratajczak, H Ulrich, A Abdel-Lati, MZ Ratajczak
Leukemia, 2018-03-30;0(0):.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Human stem cells express pannexins
Authors: N Hainz, A Beckmann, M Schubert, A Haase, U Martin, T Tschernig, C Meier
BMC Res Notes, 2018-01-22;11(1):54.
Species: Human
Sample Types: Protein
Applications: Western Blot