Human/Mouse/Rat PDHX Antibody

Catalog #: AF6014
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6014
AF6014-SP

Detection of Human, Mouse, and Rat PDHX by Western Blot.

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All PDHX Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PDHX Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat PDHX in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PDHX
aa 387-501
Accession # P15374

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat PDHX by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, mouse heart tissue, and rat heart tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PDHX Polyclonal Antibody (Catalog # AF6014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PDHX at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDHX

UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26‑28 kDa member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH-L3 cleaves both monomeric ubiquitin (Ub) from Ub-protein conjugates, and a GGLRQ peptide from the C-terminus of the Ub-like protein NEDD-8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230 amino acids (aa) in length. It is phosphorylated on Ser75 and Ser130, and contains two ubiquitin-binding sequences between aa 40‑57 and 178‑186. Four potential splice forms have been reported. Two show a four aa deletion between aa 15‑18, with one also containing a 14 aa substitution for aa 179 - 230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa 143‑230. Full-length human UCH-L3 shares 98% aa identity with mouse and rat UCH-L3.

Long Name

Pyruvate Dehydrogenase Complex, Component X

Entrez Gene IDs

8050 (Human); 27402 (Mouse); 311254 (Rat)

Alternate Names

Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenasecomplex; DLDBP; E3-binding protein; E3BP; E3BP1; E3BPpyruvate dehydrogenase complex, E3-binding protein subunit; OPDX; PDHX; PDX1; PDX1Lipoyl-containing pyruvate dehydrogenase complex component X; proX; proXpyruvate dehydrogenase complex, lipoyl-containing component X; pyruvate dehydrogenase complex, component X; pyruvate dehydrogenase protein X component, mitochondrial

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat PDHX Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Pyruvate dehydrogenase operates as an intramolecular nitroxyl generator during macrophage metabolic reprogramming
Authors: Palmieri, EM;Holewinski, R;McGinity, CL;Pierri, CL;Maio, N;Weiss, JM;Tragni, V;Miranda, KM;Rouault, TA;Andresson, T;Wink, DA;McVicar, DW;
Nature communications
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot