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Human/Mouse/Rat Phospho-CREB (S133) Antibody

Catalog #: AF2510
8 Citations Datasheet / COA / SDS

Discontinued Product

AF2510 has been discontinued.
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Detection of Human Phospho-CREB (S133) by Western Blot.
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Product Details
Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Phospho-CREB (S133) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat CREB when phosphorylated at S133 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human, mouse, and rat CREB S133 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-CREB (S133) antibody by Western Blot. View Larger

Detection of Human Phospho-CREB (S133) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CREB (S133) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2510), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CREB (S133) at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Phospho-CREB (S133) antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Phospho-CREB (S133) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-CREB (S133) at approximately 54 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CREB (S133) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2510). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CREB

The cAMP response element binding protein (CREB) belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. When phosphorylated at Ser133, CREB also binds the coactivator CREB binding protein (CBP), which enhances transcription by acetylating histones to facilitate chromatin unraveling.

Long Name
cAMP Response Element-binding Protein
Entrez Gene IDs
1385 (Human); 12912 (Mouse)
Alternate Names
active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein

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Citations for Human/Mouse/Rat Phospho-CREB (S133) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Circular RNA circHIPK3 modulates autophagy via MIR124-3p-STAT3-PRKAA/AMPK? signaling in STK11 mutant lung cancer
    Authors: X Chen, R Mao, W Su, X Yang, Q Geng, C Guo, Z Wang, J Wang, LA Kresty, DG Beer, AC Chang, G Chen
    Autophagy, 2019-06-28;0(0):1-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer.
    Authors: Dumitru CA, Fechner MK, Hoffmann TK
    J. Leukoc. Biol., 2012-01-18;91(4):591-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Mal mediates TLR-induced activation of CREB and expression of IL-10.
    Authors: Mellett M, Atzei P, Jackson R, O'Neill LA, Moynagh PN
    J. Immunol., 2011-03-11;186(8):4925-35.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  4. Soluble EP2 neutralizes prostaglandin E2-induced cell signaling and inhibits osteolytic tumor growth.
    Authors: Takahashi T, Uehara H, Bando Y, Izumi K
    Mol. Cancer Ther., 2008-09-01;7(9):2807-16.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. The kinase p38 alpha serves cell type-specific inflammatory functions in skin injury and coordinates pro- and anti-inflammatory gene expression.
    Authors: Kim C, Sano Y, Todorova K, Carlson BA, Arpa L, Celada A, Lawrence T, Otsu K, Brissette JL, Arthur JS, Park JM
    Nat. Immunol., 2008-09-01;9(9):1019-27.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP, Western Blot
  6. cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
    Authors: Siddappa R, Martens A, Doorn J, Leusink A, Olivo C, Licht R, van Rijn L, Gaspar C, Fodde R, Janssen F, van Blitterswijk C, de Boer J
    Proc. Natl. Acad. Sci. U.S.A., 2008-05-19;105(20):7281-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Antiinflammatory cAMP signaling and cell migration genes co-opted by the anthrax bacillus.
    Authors: Kim C, Wilcox-Adelman S, Sano Y, Tang WJ, Collier RJ, Park JM
    Proc. Natl. Acad. Sci. U.S.A., 2008-04-21;105(16):6150-5.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Proteinase-activated receptor-2 induces cyclooxygenase-2 expression through beta-catenin and cyclic AMP-response element-binding protein.
    Authors: Wang H, Wen S, Bunnett NW, Leduc R, Hollenberg MD, MacNaughton WK
    J. Biol. Chem., 2007-10-25;283(2):809-15.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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