Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

Catalog #: AF5126
16 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5126
AF5126-SP

Detection of Human and Rat Phospho-FRS2 (Y436) by Western Blot.

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Product Details

Citations (16)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat FRS2 isoforms when phosphorylated at Y436.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human FRS2 Y436 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Rat Phospho-FRS2 (Y436) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta -NGF (Catalog # 556-NG) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Bands were detected for Phospho-FRS2 (Y436) at approximately 70 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Phospho-FRS2 (Y436) in A431 Human Cell Line. FRS2 phosphorylated at Y436 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FRS2

FRS2 (FGF R substrate 2; also known as SNT and FRS2-alpha ) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF and RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain that interacts with FGF and NGF receptors (aa 13‑115), and a C-terminal tyrosine-rich region that serves as a docking site for GRB-2 and SHP-2 (aa 196‑471). Phosphorylation of Y436 by activated RTKs is required for efficient SHP-2 recruitment.

Long Name

Fibroblast Growth Factor Receptor Substrate 2

Entrez Gene IDs

10818 (Human); 327826 (Mouse)

Alternate Names

FGFR signalling adaptor; FGFR-signaling adaptor SNT; fibroblast growth factor receptor substrate 2; FRS2; FRS2A; FRS2alpha; SNT; SNT1; SNT-1FGFR substrate 2; Suc1-associated neurotrophic factor target 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Altered paracrine signaling from the injured knee joint impairs postnatal long bone growth
Authors: Alberto Roselló-Díez, Daniel Stephen, Alexandra L Joyner
eLife
Activated FGFR2 signalling as a biomarker for selection of intrahepatic cholangiocarcinoma patients candidate to FGFR targeted therapies
Authors: Brandi, G;Relli, V;Deserti, M;Palloni, A;Indio, V;Astolfi, A;Serravalle, S;Mattiaccio, A;Vasuri, F;Malvi, D;Deiana, C;Pantaleo, MA;Cescon, M;Rizzo, A;Katoh, M;Tavolari, S;
Scientific reports
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry
Heparin is essential for optimal cell signaling by FGF21 and for regulation of betaKlotho cellular stability
Authors: SJ An, J Mohanty, F Tome, Y Suzuki, I Lax, J Schlessing
Proceedings of the National Academy of Sciences of the United States of America, 2023-02-06;120(7):e2219128120.
Species: Chinese Hamster, Rat
Sample Types: Cell Lysates
Applications: Western Blot
Isoform-specific inhibition of FGFR signaling achieved by a de-novo-designed mini-protein
Authors: JS Park, J Choi, L Cao, J Mohanty, Y Suzuki, A Park, D Baker, J Schlessing, S Lee
Cell Reports, 2022-10-25;41(4):111545.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FGF19 Is Coamplified With CCND1 to Promote Proliferation in Lung Squamous Cell Carcinoma and Their Combined Inhibition Shows Improved Efficacy
Authors: Yanshuang Zhang, Tingyu Wu, Fan Li, Yirui Cheng, Qing Han, Xin Lu et al.
Frontiers in Oncology
Preclinical characterization of bemarituzumab, an anti-FGFR2b antibody for the treatment of cancer
Authors: Hong Xiang, Abigael G. Chan, Ago Ahene, David I. Bellovin, Rong Deng, Amy W. Hsu et al.
mAbs
INCB054828 (pemigatinib), a potent and selective inhibitor of fibroblast growth factor receptors 1, 2, and 3, displays activity against genetically defined tumor models
Authors: PCC Liu, H Koblish, L Wu, K Bowman, S Diamond, D DiMatteo, Y Zhang, M Hansbury, M Rupar, X Wen, P Collier, P Feldman, R Klabe, KA Burke, M Soloviev, C Gardiner, X He, A Volgina, M Covington, B Ruggeri, R Wynn, TC Burn, P Scherle, S Yeleswaram, W Yao, R Huber, G Hollis
PLoS ONE, 2020-04-21;15(4):e0231877.
Species: Mouse
Sample Types: Cell Culture Media
Applications: Western Blot
Fibroblast growth factor receptor inhibition induces loss of matrix MCL1 and necrosis in cholangiocarcinoma
Authors: Ayano Kabashima, Petra Hirsova, Steven F. Bronk, Matthew C. Hernandez, Mark J. Truty, Sumera Rizvi et al.
Journal of Hepatology
Gambogenic acid inhibits fibroblast growth factor receptor signaling pathway in erlotinib-resistant non-small-cell lung cancer and suppresses patient-derived xenograft growth
Authors: L Xu, X Meng, N Xu, W Fu, H Tan, L Zhang, Q Zhou, J Qian, S Tu, X Li, Y Lao, H Xu
Cell Death Dis, 2018-02-15;9(3):262.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
beta 1‐integrin controls cell fate specification in early lens development
Authors: Mallika Pathania, Yan Wang, Vladimir N. Simirskii, Melinda K. Duncan
Differentiation

Estrogen mediated epithelial proliferation in the uterus is directed by stromal Fgf10 and Bmp8a
Authors: Daesuk Chung, Fei Gao, Anil G. Jegga, Sanjoy K. Das
Molecular and Cellular Endocrinology

Role of DNA methylation and epigenetic silencing of HAND2 in endometrial cancer development.
Authors: Jones A, Teschendorff A, Li Q, Hayward J, Kannan A, Mould T, West J, Zikan M, Cibula D, Fiegl H, Lee S, Wik E, Hadwin R, Arora R, Lemech C, Turunen H, Pakarinen P, Jacobs I, Salvesen H, Bagchi M, Bagchi I, Widschwendter M
PLoS Med, 2013-11-12;10(11):e1001551.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Activation of the FGFR-STAT3 pathway in breast cancer cells induces a hyaluronan-rich microenvironment that licenses tumor formation.
Authors: Bohrer, Laura R, Chuntova, Pavlina, Bade, Lindsey, Beadnell, Thomas C, Leon, Ronald P, Brady, Nicholas, Ryu, Yungil, Goldberg, Jodi E, Schmechel, Stephen, Koopmeiners, Joseph S, McCarthy, James B, Schwertfeger, Kathryn
Cancer Res, 2013-11-06;74(1):374-86.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: IHC, Neutralization

Msx homeobox genes critically regulate embryo implantation by controlling paracrine signaling between uterine stroma and epithelium.
Authors: Nallasamy S, Li Q, Bagchi MK, Bagchi IC
PLoS Genet., 2012-02-23;8(2):e1002500.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

The function of FGF signaling in the lens placode.
Authors: Garcia CM, Huang J, Madakashira BP, Liu Y, Rajagopal R, Dattilo L, Robinson ML, Beebe DC
Dev. Biol., 2011-01-09;351(1):176-85.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Sef is a negative regulator of fiber cell differentiation in the ocular lens
Authors: Peter Newitt, Jessica Boros, Bhavani P. Madakashira, Michael L. Robinson, Lixing W. Reneker, John W. McAvoy et al.
Differentiation