Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1336) Antibody

Catalog #: PPS057 Datasheet / COA / SDS

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PPS057

Detects Phospho-GRIN2B/NMDAR2B (Y1336) by Western Blot.

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Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1336) Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Human, mouse, and rat ~180 kDa NMDA Receptor NR2B subunit phosphorylated at Y1336 in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y1336 of NMDA R, NR2B Subunit

Formulation

100 mL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 mg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detects Phospho-GRIN2B/NMDAR2B (Y1336) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDAR phosphorylated at Y1336 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l PPase). The blot is identical to the control except that it was incubated in l PPase (1200 units for 30 minutes) before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1336). The immunolabeling is completely eliminated by treatment with l PPase.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.

Background: GRIN2B/NMDAR2B

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics and mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The NR2B subunit generates a high conductance NMDAR. Human NR2B (also NR3;GRIN2B and GluRe2) is a 180-200 kDa, 1458 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 531 aa N-terminal extracellular domain (ECD), and a 646 aa cytoplasmic region. At least three tyrosines are known to be phosphorylated by Fyn in the cytoplasmic region. These include Y1252, Y1336 and Y1472. While much is known about Y1472, little information is available about the consequences of Y1252 and Y1336 phosphorylation.

References

Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.
Hess, S.D. et al. (1996) J. Pharmacol. Exp. Ther. 278:808.
Nakazawa, T. et al. (2001) J. Biol. Chem. 276:693.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B

Entrez Gene IDs

2904 (Human)

Alternate Names

DEE101;GluN1;Glutamate receptor;Glutamate receptor ionotropic, NMDA 1;GRIN1;MRD8;NDHMSD;NDHMSR;NMDA1;NMDAR1;NMD-R1;NR1; GRIN2B; NMDA R, NR2B Subunit; NMDAR2B; NR2B