Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody

Catalog #: PPS014
2 Citations Datasheet / COA / SDS

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PPS014

Detection of Phospho-GRIN2B/NMDAR2B (Y1472) by Western Blot

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Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Human, mouse, rat ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Y1472

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y1472 of NMDA R, NR2B subunit

Formulation

100 μL in 150 mM NaCl, 10 mM HEPES, 100 μg/mL BSA and 50% glycerol, pH 7.5.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Phospho-GRIN2B/NMDAR2B (Y1472) by Western Blot Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1472). The immunolabeling is completely eliminated by treatment with lambda -PPase.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GRIN2B/NMDAR2B

Rat NMDA receptor subunit 2B (NR2B) is a 1456 amino acid, 180 kDa, 3-transmembrane member of the glutamate-gated ion channel family. NMDA are heteromultimers of at least two NR1 subunits and two NR2 or two (presumably) NR3 subunits. Upon ligand binding, NMDA receptors open and allow cation transit. The NR1 subunits bind glycine and are essential for the physical formation of the ion channel. NR2 subunits apparently bind glutamate and impart pharmacological properties to the receptor complex. Fully functional receptors require the presence of both glycine and glutamate. The NR1-NR2B complex has the highest affinity for glutamate. Both NR1 and NR2B subunits can be phosphorylated by PKC. On NR1, PKC phosphorylates on serine at 890; on NR2B, PKC phosphorylates on tyrosine at 1472. Phosphorylation is considered to promote ion transit and impact NR2B interaction with downstream signaling molecules.

References

Grosshans, D.R. and M.D. Browning (2001) J. Neurochem. 76:737.
Cheung, H. et al. (2003) J. Neurochem. 86:1441.
Gibb, A.J. (2004) Trends Neurosci. 27:7.
Liu, Y. and J. Zhang (2000) Chin. Med. J. 113:948.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B

Entrez Gene IDs

2904 (Human)

Alternate Names

DEE101;GluN1;Glutamate receptor;Glutamate receptor ionotropic, NMDA 1;GRIN1;MRD8;NDHMSD;NDHMSR;NMDA1;NMDAR1;NMD-R1;NR1; GRIN2B; NMDA R, NR2B Subunit; NMDAR2B; NR2B

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Citations for Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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