Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antibody

Name: Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antibody
Brand: R&D Systems
SKU: AF889

Catalog #: AF889
1 Citation Datasheet / COA / SDS

Discontinued Product

AF889 has been discontinued.

View all RSK products.

Detection of Human Phospho-RSK (S380) by Western Blot.

4 Images

All RSK Antibodies

Product Details

Citations (1)

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat RSK isoforms when phosphorylated at sites corresponding to S380 of human RSK1.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human RSK1 S380 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human Phospho-RSK (S380) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA in the presence or absence of the indicated concentrations of the MEK1/2 inhibitor U0126 for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF889), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK (S380) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

View Larger

Detection of Human Phospho-RSK (S380) by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20, loaded at 0.2 mg/mL. A specific band was detected for Phospho-RSK (S380) at approximately 90 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF889). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

View Larger

Phospho-RSK (S380) Pan Specific in Mouse Embryo. RSK phosphorylated at S380 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF889) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to retinal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

View Larger

Phospho-RSK (S380) Pan Specific in Mouse Embryo. RSK phosphorylated at S380 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF889) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to mesenchyme. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RSK

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.

Long Name

Ribosomal Protein S6 Kinase II

Alternate Names

HU-1;MAPKAPK1;MAPKAPK1A;p90Rsk;Ribosomal protein S6 kinase alpha-1;RPS6KA1;RSK;RSK1; RSK

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

mTORC1-activated S6K1 phosphorylates Rictor on threonine 1135 and regulates mTORC2 signaling.
Authors: Julien LA, Carriere A, Moreau J, Roux PP
Mol. Cell. Biol., 2009-12-07;30(4):908-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot