Human/Mouse/Rat PIM2 Antibody Summary
Met1-Pro311
Accession # Q9P1W9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PIM2 by Western Blot. Western blot shows Recombinant Human Active PIM1 (Catalog # 4588-KS), recombinant human PIM2, and recombinant human PIM3 (5 ng/lane). PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/ Mouse/Rat PIM2 Monoclonal Antibody (Catalog # MAB4355) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PIM2 was detected at approximately 38-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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Detection of Human/Mouse/Rat PIM2 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, SW480 human colorectal adenocarcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat PIM2 Monoclonal Antibody (Catalog # MAB4355) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PIM2 was detected at approximately 38 kDa and 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIM2
The three members of the Pim family of proto-oncogenes encode the serine/threonine kinases PIM1, PIM2, and PIM3. Multiple isoforms of PIM2 are expressed in many tissues, with high levels in spleen, thymus, testis, small intestine, and colon. PIM2 is implicated as a mediator of IL-3 survival signaling in hematopoietic cells, and dysregulated PIM2 expression is observed in prostate cancer, lymphoma, leukemia, and multiple myeloma.
Product Datasheets
Citations for Human/Mouse/Rat PIM2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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High ETV6 Levels Support Aggressive B Lymphoma Cell Survival and Predict Poor Outcome in Diffuse Large B-Cell Lymphoma Patients
Authors: D Marino, M Pizzi, I Kotova, R Schmidt, C Schröder, V Guzzardo, I Talli, E Peroni, S Finotto, G Scapinello, AP Dei Tos, F Piazza, L Trentin, V Zagonel, E Piovan
Cancers, 2022-01-11;14(2):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Role of PIM2 in allergic asthma
Authors: W Du, T Chen, Y Ni, X Hou, Y Yu, Q Zhou, F Wu, W Tang, G Shi
Mol Med Rep, 2017-09-18;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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