Human/Mouse/Rat PKA C alpha Antibody

Name: Human/Mouse/Rat PKA C alpha Antibody
Brand: R&D Systems
SKU: AF4268
Rating: 5 (1 reviews)

Catalog #: AF4268 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4268
AF4268-SP

Detection of Human/Mouse/Rat PKA C alpha by Western Blot.

1 Image

All PKA C alpha Antibodies

Product Details

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PKA C alpha Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat PKA C alpha in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) PKA C beta or rhPKA Cδ is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PKA C alpha
Met1-Phe351
Accession # P17612

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human/Mouse/Rat PKA C alpha by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Mouse/Rat PKA Ca Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active PKA C alpha (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (3 ng/lane) were included. A specific band for PKA C alpha was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKA C alpha

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha, C beta, and C gamma, have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit alpha

Entrez Gene IDs

5566 (Human); 18747 (Mouse); 25636 (Rat)

Alternate Names

cAMP-dependent protein kinase catalytic subunit alpha; Cs; EC 2.7.11; EC 2.7.11.11; MGC102831; MGC48865; PKA C alpha; PKA C-alpha; Pkaca; PKCA1; PKCD; PRKACA; protein kinase A catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha