Human/Mouse/Rat PNUTS Antibody

Catalog #: AF21581 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF21581
AF21581-SP

Detection of Mouse and Rat PNUTS by Western Blot.

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Human/Mouse/Rat PNUTS Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat PNUTS in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PNUTS
Pro5-Asn226
Accession # NP_002705

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse and Rat PNUTS by Western Blot. Western blot shows lysates of TS1 mouse helper T cell line and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat PNUTS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21581) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PNUTS at approximately 114 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Simple Western

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Detection of Human PNUTS by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for PNUTS at approximately 100-150 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat PNUTS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF21581). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PNUTS

PP1 nuclear targeting subunit (PNUTS), also known as PPP1R10 and FB19, is a 99 kDa protein that binds the serine/threonine protein phosphatase PP1 and causes its localization to chromatin in the nucleus. C-terminal RGG repeats in PNUTS suggest it may bind to single stranded RNA. The PP1/PNUTS complex may also play a role in regulating the cell cycle-dependent phosphorylation of the oncogene Rb.

Long Name

Phosphatase-1 Nuclear Targeting Subunit

Entrez Gene IDs

5514 (Human); 52040 (Mouse); 65045 (Rat)

Alternate Names

CAT53; FB19PP1-binding protein of 114 kDa; MHC class I region proline-rich protein CAT53; p99; Phosphatase 1 nuclear targeting subunit; phosphatase nuclear targeting subunit; PNUTS; PNUTSPP1R10; PPP1R10; Protein FB19; protein phosphatase 1 regulatory subunit 10; protein phosphatase 1, regulatory (inhibitor) subunit 10; protein phosphatase 1, regulatory subunit 10; serine/threonine-protein phosphatase 1 regulatory subunit 10