Human/Mouse/Rat PSMA2 Antibody

Catalog #: MAB7639 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7639
MAB7639-SP

Detection of Human, Mouse, and Rat PSMA2 by Western Blot.

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All PSMA2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PSMA2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects Human/Mouse/Rat PMSA2 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 797016

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PMSA2
Met1-Ala234
Accession # P25787

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat PSMA2 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat PSMA2 Monoclonal Antibody (Catalog # MAB7639) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PSMA2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSMA2

PSMA2 (Proteasome subunit alpha type 2; also known as proteasome component C3 and Macropain subunit C3) is an intracellular 25-27 kDa member of the peptidase T1A family of molecules. It is ubiquitously expressed, and participates in the formation of the 700 kDa 20S proteasomal complex. This complex contains four stacked rings, forming a barrel. The top and bottom rings are made of seven alpha -subunits, while the two central rings are composed of seven catalytic beta -subunits. The complex degrades 80-90% of all intracellular proteins through both an ubiquitin-dependent and -independent process. PSMA2 may modulate the activity of select beta -subunits. Human PSMA2 is 234 amino acids (aa) in length. It contains at least five utilized phosphorylation sites plus one nitrated tyrosine. Full-length human PSMA2 shares 99% aa sequence identity with mouse PSMA2.

Long Name

Proteasome (Prosome, Macropain) Subunit, alpha Type, 2

Entrez Gene IDs

5683 (Human); 19166 (Mouse); 29669 (Rat)

Alternate Names

HC3; HC3;MU;PMSA2;Proteasome subunit alpha type;Proteasome subunit alpha type-2;PSC2; MU; PMSA2; PSC3; PSMA2