Human/Mouse/Rat Rab11A Antibody

Name: Human/Mouse/Rat Rab11A Antibody
Brand: R&D Systems
SKU: MAB8978
Rating: 3 (1 reviews)

Catalog #: MAB8978 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8978
MAB8978-SP

Detection of Human, Mouse, and Rat Rab11A by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Rab11A Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Rab-11A in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 918009

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Rab-11A
Met1-Glu162
Accession # P62491

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Rab11A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HT1080 human fibrosarcoma cell line, C2C12 mouse myoblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Rab11A Monoclonal Antibody (Catalog # MAB8978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Rab11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rab11A

Ras-related protein Rab11A is a 25 kDa member of the Rab family of small GTPases. With more than 40 family members, Rab proteins are regulators of vesicular transport, and their number reflects the complexity of vesicle trafficking. Rab family members cycle between an inactive GDP-bound form and an active GTP-bound form that recruits to membranes various downstream effectors directly responsible for vesicle formation, migration and fusion. Rab11A is ubiquitously expressed, with Northern blot analysis detecting highest levels in heart, and lowest levels in liver. Human Rab11A is 216 amino acids (aa) in length, with residues 214-216 removed in its mature form. Over aa 1-162, human Rab11A shares 100% identity with mouse and rat Rab11A.

Long Name

RAs Genes from Brain Protein 11A

Entrez Gene IDs

8766 (Human); 53869 (Mouse); 81830 (Rat)

Alternate Names

MGC1490; RAB 11A, member oncogene family; RAB11; Rab-11; Rab11A; RAB11A, member RAS oncogene family; YL8; YL8ras-related protein Rab-11A