Human/Mouse/Rat RBBP4 Antibody

Catalog #: MAB7416
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7416-100
MAB7416-SP

Detection of Human, Mouse, and Rat RBBP4 by Western Blot.

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All RBBP4 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat RBBP4 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human RBBP-4 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 968701

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human RBBP-4
Ala2-Ser425
Accession # 09028

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat RBBP4 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Neuro-2A mouse neuroblastoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat RBBP4 Monoclonal Antibody (Catalog # MAB7416) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for RBBP4 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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RBBP4 in Human Breast Cancer Tissue. Retinoblastoma Binding Protein 4 (RBBP4) was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse/Rat RBBP4 Monoclonal Antibody (Catalog # MAB7416) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RBBP4

RBBP4 (Retinoblastoma-Binding Protein 4; also RbAp48 and CAF-1) is a 48-56 kDa member of the WD Repeat RBAP46/48/MSI1 family of proteins. It is ubiquitously expressed, and acts as a transcriptional repressor. At the start of G1 of the cell cycle, Rb (retinoblastoma protein) normally associates with an E2F transcription complex on E2F responsive genes, blocking E2F activity. At the appropriate time, Rb is phosphorylated, causing its dissociation from E2F and resulting in E2F activation. Dephosphorylated Rb apparently mediates transcriptional repression by recruiting a histone deacetylase (HDAC), followed by HDAC binding to RBBP4. RBBP4, being a histone-binding protein, now brings histones plus HDACs together, resulting in histone deacetylation and gene silencing. Human RBBP4 is 425 amino acids (aa) in length. It contains six WD repeats (aa 122-403), two N-terminal acetylation sites, and two serine phosphorylation sites at positions 110 and 146. There are at least two isoform variants. One contains a six aa substitution for aa 405-425, while another utilizes an alternative start site at Met36. Human and mouse RBBP4 are identical in aa sequence.

Long Name

Retinoblastoma Binding Protein 4

Entrez Gene IDs

5928 (Human); 19646 (Mouse); 685491 (Rat)

Alternate Names

CAF-1 p48; CAF-1 subunit C; CAF-1C; CAF-I p48; Chromatin assembly factor 1 subunit C; Chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; histone-binding protein RBBP4; MSI1 protein homolog; Nucleosome-remodeling factor subunit RBAP48; NURF55; RBAP48; RBBP4; RBBP-4; retinoblastoma binding protein 4; Retinoblastoma-binding protein 4CAF-I 48 kDa subunit; Retinoblastoma-binding protein p48

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat RBBP4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Direct interaction between the PRDM3 and PRDM16 tumor suppressors and the NuRD chromatin remodeling complex
Authors: D Ivanochko, L Halabelian, E Henderson, P Savitsky, H Jain, E Marcon, S Duan, A Hutchinson, A Seitova, D Barsyte-Lo, P Filippakop, J Greenblatt, E Lima-Ferna, CH Arrowsmith
Nucleic Acids Res., 2019-02-20;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot