Human/Mouse/Rat RSK Pan Specific Antibody

Catalog #: AF2056
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2056
AF2056-SP

Detection of Human and Mouse RSK by Western Blot.

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Citations (2)

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Human/Mouse/Rat RSK Pan Specific Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat RSK family isoforms in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Human RSK2 synthetic peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse RSK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat RSK Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2056) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RSK Pan Specific, at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RSK

This peptide sequence is identical to amino acids 162‑184 of human RSK3 (RPS6KA2). The sequence differs at one position from amino acids 165‑187 of human RSK1 (RPS6KA1) and at two positions from amino acids 176‑198 of human RSK4 (RPS6KA6). The immunogen is also highly homologous to the corresponding sequences of mouse and rat RSK family isoforms.

Long Name

Ribosomal Protein S6 Kinase II

Alternate Names

HU-1;MAPKAPK1;MAPKAPK1A;p90Rsk;Ribosomal protein S6 kinase alpha-1;RPS6KA1;RSK;RSK1; RSK

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat RSK Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Molecular imaging of the kinetics of hyperactivated ERK1/2-mediated autophagy during acquirement of chemoresistance
Authors: A Bishnu, P Phadte, A Dhadve, A Sakpal, B Rekhi, P Ray
Cell Death & Disease, 2021-02-08;12(2):161.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Integrins promote cytokinesis through the RSK signaling axis.
Authors: Mathew S, Nieves B, Sequeira S, Sambandamoorthy S, Pumiglia K, Larsen M, Laflamme S
J Cell Sci, 2013-11-27;127(0):534-45.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot